Seqüenciació de nova generació pel genotipat del VIH
GINYS-IRSICAIXA-001
Teresa Puig
Responsable
La determinació de resistències genotípiques als antiretrovirals es una eina de gran utlilitat en l'optimitzacio i seguiment del tractament antidretroviral dels pacients VIH positius.
El servei es va posar en marxa al 1999, i l’any 2002 el Servei Català de la Salut el va designar centre de referència per al test de resistències als antiretrovirals. En paral·lel a aquesta tasca assistencial, també participa en projectes de recerca i assajos clínics en col·laboració amb altres institucions i companyies farmacèutiques.
Serveis
Test de resistencies genotipiques als antiretrovirals mitjançant NGS.
Posibilitat de realitzar el test en mostres de baixa carrega viral mitjançant la concentracio de la mostra per ultracentifugacio.
Equipament
NSG Worflow
- Sentosa SX101
- Sentosa ST401i i ST401e
- Sentosa SQ301
- Sentosa SQ Reporter
Miniplate spinner
Pipetes
Publicacions
Comparison of the LiPA HIV-1 RT test, selective PCR and direct solid phase sequencing for the detection of HIV-1 drug resistance mutations.
J Virol Methods . 1998 Jul;73(1):77-82. doi: 10.1016/s0166-0934(98)00043-3
J Virol Methods . 1998 Jul;73(1):77-82. doi: 10.1016/s0166-0934(98)00043-3
Evolution of drug resistance in HIV-infected patients remaining on a virologically failing combination antiretroviral therapy regimen
AIDS . 2007 Mar 30;21(6):721-32. doi: 10.1097/QAD.0b013e3280141fdf
AIDS . 2007 Mar 30;21(6):721-32. doi: 10.1097/QAD.0b013e3280141fdf
Prevalence of transmitted antiretroviral resistance and distribution of HIV-1 subtypes among patients with recent infection in Catalonia (Spain) between 2003 and 2005.
Enferm Infecc Microbiol Clin . 2011 Aug-Sep;29(7):482-9. doi: 10.1016/j.eimc.2011.03.001. Epub 2011 May 2
Enferm Infecc Microbiol Clin . 2011 Aug-Sep;29(7):482-9. doi: 10.1016/j.eimc.2011.03.001. Epub 2011 May 2
Prevalence of genotypic resistance to nucleoside analogues and protease inhibitors in Spain. The ERASE-2 Study Group
AIDS. 2000 Apr 14;14(6):727-32. doi: 10.1097/00002030-200004140-00012. PMID: 10807196
AIDS. 2000 Apr 14;14(6):727-32. doi: 10.1097/00002030-200004140-00012. PMID: 10807196
Evaluation of the Innate Immune Modulator Acitretin as a Strategy To Clear the HIV Reservoir
Antimicrob Agents Chemother. 2017 Oct 24;61(11):e01368-17. Print 2017 Nov. PMID: 28874382
Antimicrob Agents Chemother. 2017 Oct 24;61(11):e01368-17. Print 2017 Nov. PMID: 28874382
RNA editing by ADAR1 regulates innate and antiviral immune functions in primary macrophages
Sci Rep. 2017 Oct 17;7(1):13339. PMID: 29042669 Free PMC article
Sci Rep. 2017 Oct 17;7(1):13339. PMID: 29042669 Free PMC article
Long-term HIV-1 infection induces an antiviral state in primary macrophages
Antiviral Res. 2016 Sep;133:145-55. Epub 2016 Aug 7. PMID: 27510577
Antiviral Res. 2016 Sep;133:145-55. Epub 2016 Aug 7. PMID: 27510577
The G1/S Specific Cyclin D2 Is a Regulator of HIV-1 Restriction in Non-proliferating Cells.
PLoS Pathog. 2016 Aug 19;12(8):e1005829. eCollection 2016 Aug. PMID: 27541004 Free PMC article
PLoS Pathog. 2016 Aug 19;12(8):e1005829. eCollection 2016 Aug. PMID: 27541004 Free PMC article
Inhibition of herpes simplex virus type 1 by the CDK6 inhibitor PD-0332991 (palbociclib) through the control of SAMHD1.
J Antimicrob Chemother. 2016 Feb;71(2):387-94. Epub 2015 Nov 4. PMID: 26542306 Free PMC article
J Antimicrob Chemother. 2016 Feb;71(2):387-94. Epub 2015 Nov 4. PMID: 26542306 Free PMC article
RECall for automated genotypic tropism testing
J Clin Microbiol. 2013 Aug;51(8):2754-7. Epub 2013 Jun 12. PMID: 23761150 Free PMC article.
J Clin Microbiol. 2013 Aug;51(8):2754-7. Epub 2013 Jun 12. PMID: 23761150 Free PMC article.
Genotypic and phenotypic evolution of HIV type-1 protease during in vitro sequential or concomitant combination of atazanavir and amprenavir
Antivir Ther. 2010;15(3):431-6. PMID: 20516562
Antivir Ther. 2010;15(3):431-6. PMID: 20516562
Viral evolution during structured treatment interruptions in chronically human immunodeficiency virus-infected individuals
J Virol. 2002 Dec;76(23):12344-8. PMID: 12414975 Free PMC article.
J Virol. 2002 Dec;76(23):12344-8. PMID: 12414975 Free PMC article.