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Mutant mouse

GINYS-IRB-005
Dr. Stephen Forrow
Core Facility Manager
stephen.forrow@irbbarcelona.org | +34 93 403 9771 | ORCID | PRC page
FACILITY WEBSITE
The facility was created in 2007. Our primary function is to generate genetically modified mouse models for users within IRB Barcelona. We are able to offer a comprehensive service for the generation of genetically altered mice including:
  • Design of recombinant DNA molecules for genetic modification.
  • Help with cloning protocols and reagents required to generate gene targeting and transgenic vectors.
  • All cell-culture required for gene targeting in mouse embryonic stem (ES) cells, including expansion, transfection, drug selection, colony picking and colony expansion.
  • Microinjection of DNA or ES cells into pre-implantation stage embryos.
  • Electroporation of DNA/mRNA/proteins into pre-implantation stage embryos.
  • Transfer of manipulated embryos into pseudo-pregnant foster mice.
  • Subsequent processing of pups born to pseudopregnant fosters.

Services

The facility has a fully equipped laboratory for cell and molecular biology. Mouse embryonic stem (ES) cell culture is carried out within a dedicated cell culture laboratory.
We also have a small microinjection suite based within the PCB Animal Research Center (SEA). This suite contains inverted microscopes equipped with state of the art micromanipulators and microinjectors. The suite also contains everything necessary for mouse surgery and embryo manipulation.
We are equipped to carry out cryopreservation of mouse germ-line tissue.

Equipment

Molecular biology equipment
Cell biology Equipment
Embryo manipulation equipment

Staff

Core Facility Manager
Dr. Stephen Forrow | stephen.forrow@irbbarcelona.org | +34934039771 | ORCID | PRC Page

Publications

BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis
Welz, Patrick-Simon, Zinna, Valentina M., Symeonidi, Aikaterini, Koronowski, Kevin B., Kinouchi, Kenichiro, Smith, Jacob G., Guillén, Inés Marín, Castellanos, Andrés, Forrow, Stephen, Aragón, Ferrán, Crainiciuc, Georgiana, Prats, Neus, Caballero, Juan Martín, Hidalgo, Andrés, Sassone-Corsi, Paolo, Benitah, Salvador Aznar
Cell 177, 1436–1447.e1–e12; May 30, 2019
DOI: 10.1016/J.CELL.2019.05.009
Differential requirements for Tousled-like kinases 1 and 2 in mammalian development
Segura-Bayona S; Knobel PA; Gonzalez-Buron H; Youssef SA; Peña-Blanco A; Coyaud E; Lopez-Rovira T; Rein K; Palenzuela L; Colombelli J; Forrow S; Raught B; Groth A; De Bruin A; Stracker TH
Cell Death And Differentiation 24 (11 ) 1872 - 1885 (2017)
DOI: 10.1038/cdd.2017.108
GEMC1 is a critical regulator of multiciliated cell differentiation
Berta Terré , Gabriele Piergiovanni, Sandra Segura-Bayona, Gabriel Gil-Gómez, Sameh A Youssef, Camille Stephan-Otto Attolini, Michaela Wilsch-Bräuninger, Carole Jung, Ana M Rojas, Marko Marjanović, Philip A Knobel, Lluís Palenzuela, Teresa López-Rovira, Stephen Forrow, Wieland B Huttner, Miguel A Valverde, Alain de Bruin, Vincenzo Costanzo, Travis H Stracker
EMBO J . 2016 May 2;35(9):942-60
DOI: embj.201592821
Hepatic overexpression of a constitutively active form of liver glycogen synthase improves glucose homeostasis
Ros S; Zafra D; Valles-Ortega J; García-Rocha M; Forrow S; Domínguez J; Calbó J; Guinovart J
Journal Of Biological Chemistry 285 (48 ) 37170 - 37177 (2010)
DOI: 10.1074/jbc.M110.157396

INSTITUTE

IRB Barcelona - Institut de Recerca Biomèdica

Parc Científic de Barcelona
Baldiri Reixac, 10

08028 Barcelona http://www.irbbarcelona.org/ca

DOMAINS RIS3CAT

  • Education and knowledge generation system
  • Industrial system
  • Socio-sanitary system

CATEGORIES

  • STABLES AND ANIMAL EXPERIMENTATION CENTERS
    • Specialized animal experimentation
    • Stables small animals

RATES AND ACCESS

Availability Level: Low
Access Procedures:
CERCA - Ginys