Histopathology
GINYS-IRB-001
The facility offers histopathological services and training opportunities to the IRB scientific community and external public and private research centers, applying their experience to provide personalized and cost-effective support.
The facility offers a wide variety of histological studies, ranging from routine techniques and services to the development of new approaches (IHC, IF, ...), experimental pathology studies, reports and consulting services (phenotyping of mouse, animal disease models, drug efficacy). and non-LPG toxicity studies).
The staff of the facilities also participates in the organization and teaching of specialization courses and master's degrees in Pathology and Toxicology of Laboratory Animals.
Services
- Histopathological studies and reports of rodents:
- Assistance in the implementation and development of the study of the protocol (experimental design).
- Mouse phenotyping
- Animal models of disease (inflammatory, autoimmune, neoplastic)
- Drug efficacy
- Toxicity of non-LPG drugs
- Necropsy of whole rodents, tissue collection, raw scan with report.
- Training in necropsy techniques, including blood collection and perfusion
- Fixation of cells, tissues and organs
- Tissue trimming, freezing and embedding for histology
- Paraffin-embedded and frozen sections
- Routine staining of hematoxylin and eosin sections
- Specialized histochemical staining of sections (e.g., Tricrom, PAS, Pearl’s, and many other spots)
- Immunohistochemistry (IHC) and immunofluorescence (IF) studies.
- Digital image scanning
- Quantitative techniques for morphological evaluation (histomorphometry, computer image analysis)
Equipment
- Tissue-Tek VIP Vacuum Infiltration Processor – Sakura
- RM2255 Rotary Microtome – Leica
- CM1950 Cryostat – Leica
- CoverStainer – Dako
- Artisan Link Pro – Dako
- AutostainerPlus – Dako
- Discovery XT window – Roche
- CX43 Biological Microscope – Oympus
- DM1000 LED bright field microscope with ICC50 HD digital camera – LEICA
- Nanozoomer 2.0HT – Hamamatsu
Staff
Publications
Defects in efferent duct multiciliogenesis underlie male infertility in GEMC1-, MCIDAS- or CCNO-deficient mice
Development
Development
Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging
Cell
Cell
A genome editing approach to study cancer stem cells in human tumors
Embo Molecular Medicine
Embo Molecular Medicine
