Histopathology
GINYS-IRB-001
Dra. Neus Prats
CORE FACILITY MANAGER
The facility offers histopathological services and training opportunities to the IRB scientific community and external public and private research centers, applying their experience to provide personalized and cost-effective support.
The facility offers a wide variety of histological studies, ranging from routine techniques and services to the development of new approaches (IHC, IF, ...), experimental pathology studies, reports and consulting services (phenotyping of mouse, animal disease models, drug efficacy). and non-LPG toxicity studies).
The staff of the facilities also participates in the organization and teaching of specialization courses and master's degrees in Pathology and Toxicology of Laboratory Animals.
Services
- Histopathological studies and reports of rodents:
- Assistance in the implementation and development of the study of the protocol (experimental design).
- Mouse phenotyping
- Animal models of disease (inflammatory, autoimmune, neoplastic)
- Drug efficacy
- Toxicity of non-LPG drugs
- Necropsy of whole rodents, tissue collection, raw scan with report.
- Training in necropsy techniques, including blood collection and perfusion
- Fixation of cells, tissues and organs
- Tissue trimming, freezing and embedding for histology
- Paraffin-embedded and frozen sections
- Routine staining of hematoxylin and eosin sections
- Specialized histochemical staining of sections (e.g., Tricrom, PAS, Pearl’s, and many other spots)
- Immunohistochemistry (IHC) and immunofluorescence (IF) studies.
- Digital image scanning
- Quantitative techniques for morphological evaluation (histomorphometry, computer image analysis)
Equipment
- Tissue-Tek VIP Vacuum Infiltration Processor – Sakura
- RM2255 Rotary Microtome – Leica
- CM1950 Cryostat – Leica
- CoverStainer – Dako
- Artisan Link Pro – Dako
- AutostainerPlus – Dako
- Discovery XT window – Roche
- CX43 Biological Microscope – Oympus
- DM1000 LED bright field microscope with ICC50 HD digital camera – LEICA
- Nanozoomer 2.0HT – Hamamatsu
Publications
Multi-omic rejuvenation of naturally aged tissues by a single cycle of transient reprogramming
Aging Cell 21 (3 ) e13578 (2022)
Aging Cell 21 (3 ) e13578 (2022)
Dietary palmitic acid promotes a prometastatic memory via Schwann cells
Nature 599 (7885 ) 485 (2021)
Nature 599 (7885 ) 485 (2021)
Pulmonary glycogen deficiency as a new potential cause of respiratory distress syndrome
Human Molecular Genetics 29 (21 ) 3554 - 3565 (2020)
Human Molecular Genetics 29 (21 ) 3554 - 3565 (2020)
BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis
Cell 177 (6 ) 1436 (2019)
Cell 177 (6 ) 1436 (2019)
Defects in efferent duct multiciliogenesis underlie male infertility in GEMC1-, MCIDAS- or CCNO-deficient mice
Development
Development
Loss of G9a preserves mutation patterns but increases chromatin accessibility, genomic instability and aggressiveness in skin tumours
Nature Cell Biology 20 (12 ) 1400 (2018)
Nature Cell Biology 20 (12 ) 1400 (2018)
Identity Noise and Adipogenic Traits Characterize Dermal Fibroblast Aging
Cell 175 (6 ) 1575 (2018)
Cell 175 (6 ) 1575 (2018)
Loss of dnmt3a and dnmt3b does not affect epidermal homeostasis but promotes squamous transformation through PPAR-g
Elife 6 e21697 (2017)
Elife 6 e21697 (2017)
A genome editing approach to study cancer stem cells in human tumors
Embo Molecular Medicine 9 (7 ) 869 - 879 (2017)
Embo Molecular Medicine 9 (7 ) 869 - 879 (2017)
Targeting metastasis-initiating cells through the fatty acid receptor CD36
Nature 541 (7635 ) 41 (2017)
Nature 541 (7635 ) 41 (2017)
