Biobank HCB-FRCB-IDIBAPS
GINYS-IDIBAPS-001
Teresa Botta Orfila, PhD
Head of Biobank HCB-FRCB-IDIBAPS
Biobanc HCB – FRCB – IDIBAPS
Centre Esther Koplowitz (CEK)
C/ Rosselló 149-153
08036 Barcelona
Centre Esther Koplowitz (CEK)
C/ Rosselló 149-153
The Biobank of the Hospital Clínic de
Barcelona - Fundació Recerca Clínic Barcelona - Institut d'Investigacions
Biomèdiques August Pi i Sunyer (HCB-FRCB-IDIBAPS Biobank) is a centralized
platform that coordinates the collection, processing, storage and procurement
of human biological samples and associated data to promote high-quality
biomedical research. These samples are available to the scientific community
with the aim of promoting personalized medicine. The Biobank is highly
specialized in sample processing, providing researchers with several technical
services to process human and animal samples. The Biobank works under the
ISO9001 quality management system.
The Biobank sample catalog is structured in three
major banks:
· Neurological Tissues Bank (BTN)
· Tumour and Tissue Bank (BTT)
· Biological Fluids Bank (BFB)
People interested in donating neurological tissue for
research in neurological diseases can find more information in this leaflet.
Services
PROVISION OF HUMAN BIOLOGICAL SAMPLES AND ASSOCIATED
DATA
DATA
– Frozen, paraffin-embedded and cryopreserved samples
of nervous tissue from donors affected by neurological diseases and control
patients
of nervous tissue from donors affected by neurological diseases and control
patients
– Frozen, paraffin-embedded and fresh samples of a
wide range of pathologies, with adjacent healthy tissue
wide range of pathologies, with adjacent healthy tissue
– DNA samples, serum, plasma and PBMCs from different diseases and
control patients
control patients
TECHNICAL AND SCIENTIFIC ADVICE
– Management of new sample collections, with national registration support
– Support on informed consent preparation
– Technical assistance on experimental design with human and animal samples
– Design and interpretation of immunohistochemical studies
– Training in facility equipments and specialized software
– Quality control programs
– Standardization of procedures for DNA handling and quality controls
PRIVATE COLLECTION MANAGEMENT
– Sample management, processing, traceability control, and custody for clinical trials
– Coordination of multicentric collections
– Sample storage and custody (-80ºC and liquid nitrogen)
HISTOLOGICAL TECHNIQUES
– Processing of frozen or paraffin-embedded tissue samples (block preparation, histological sections, macrodissection)
– Neurological tissue processing and post-mortem neuropathological evaluation
– Support with laser microdissection
– Preparation of Tissue Microarrays (TMAs)
– Histological stains
– Scanning of histological preparations and pathological evaluation
SAMPLE PROCESSING TECHNIQUES
– Serum and plasma isolation
– Purification and cryopreservation of PBMCs and granulocytes
– Automated services:
· nucleic acid isolation (fluid and solid samples)
· aliquoting of fluid samples (serum, plasma, urine, buffy coat)
· immunohistochemistry
Equipment
The HCB – FRCB – IDIBAPS Biobank features automated equipment that allows sample traceability and quality control, as well as the development of innovative and useful techniques. The most relevant equipment are the following:
· Three automated DNA extraction platforms;
· An aliquoting station for fluid samples (such as blood, plasma, serum urine or DNA);
· Two paraffin processors for tissue inclusion;
· Two automated immunohistochemistry platforms;
· A TMA platform;
· A high-resolution scanner for histological preparations;
· A laser microdissector;
· A sample selector (Mohawk Tube Picker);
The Biobank also features other equipment such as two multihead microscopes, spectrophotometers, microtomes and cryostats, as well as a fully equipped culture room with four laminar flux cabinets, an incubator, a microscope, a freezing ramp and four centrifuges.
The facility counts with thirty-nine -80ºC ultrafreezers and six liquid nitrogen tanks, as well as a several refrigerators and -20ºC freezers. Moreover, sample and data are handled through NorayBanks information system and the HCB SAP system.
Staff
Aina Rodríguez Vilarrupla, PhD, Biobank HCB-FRCB-IDIBAPS Scientific Director, Core Facilities Director | aina.rodriguez@idibaps.org
| ORCID
| PRC Page
Teresa Botta Orfila, PhD, Head of Biobank HCB-FRCB-IDIBAPS Core Facility and Freezing and Criopreservation Service | mbotta@recerca.clinic.cat
| ORCID
Gemma Aragonès Bargalló, PhD, Biobank Area Coordinator | garagones@recerca.clinic.cat
Iban Aldecoa Ansorregui, MD, PhD, Neuropathologist | ialdecoa@clinic.cat
Laura Molina Porcel, MD, PhD, Neurologist | lmolinap@clinic.cat
Verónica Santiago Valera, Laboratory Technician | vsantiag@recerca.clinic.cat
Noemi González Abuín, PhD, Biobank Area Coordinator | ngonzaleza@recerca.clinic.cat
| ORCID
Cristina Merino Zamorano, PhD, Senior Laboratory Technician | cmerino@recerca.clinic.cat
Laura Gelabert Blesa, Laboratory Technician | lgelaber@recerca.clinic.cat
Mònica Marín Peñalver, Laboratory Technician | mmarin2@recerca.clinic.cat
Mercè Bonastre Garcia, Laboratory Technician | bonastre@recerca.clinic.cat
Ada Soler Ventura, PhD, Biobank Area Coordinator | adsoler@recerca.clinic.cat
| ORCID
Cèlia Segura Torres, MSc, Senior Laboratory Technician | csegura@recerca.clinic.cat
Roser Mas Malavila, MSc, Laboratory Technician | rmas1@recerca.clinic.cat
Andrea Monfort Querol, MSc, Laboratory Technician | anmonfort@recerca.clinic.cat
Aida Hernández Román, MSc, Laboratory Technician (Freezing and Criopreservation) | aihernandez@recerca.clinic.cat
Ángela Martín Cordobés, Documentation Technician | martin3@recerca.clinic.cat
Judith Sabaté del Río, MSc, Data Manager | jusabate@recerca.clinic.cat
Anna Sastre Rodó, Quality Management System coordinator | sastre@recerca.clinic.cat
Tatiana Isabel Moreano Vitor, Data Manager | moreano@recerca.clinic.cat
Núria Peiró Gabà, MSc, Senior Laboratory Technician | npeiro@recerca.clinic.cat
Publications
Evolution of Clinical-Pathological Correlations in Early-Onset Alzheimer’s Disease Over a 25-Year Period in an Academic Brain Bank
J Alzheimers Dis. 2022;87(4):1659-1669. doi: 10.3233/JAD-220045. PMID: 35723108
J Alzheimers Dis. 2022;87(4):1659-1669. doi: 10.3233/JAD-220045. PMID: 35723108
Assessment of Cognitive Symptoms in Brain Bank-Registered Control Subjects: Feasibility and Utility of a Telephone-Based Screening
J Alzheimers Dis. 2022;85(3):1107-1113. doi: 10.3233/JAD-215444. PMID: 34924396
J Alzheimers Dis. 2022;85(3):1107-1113. doi: 10.3233/JAD-215444. PMID: 34924396
Sporadic Creutzfeldt-Jakob disease VM1: phenotypic and molecular characterization of a novel subtype of human prion disease
Acta Neuropathol Commun. 2022 Aug 17;10(1):114. doi: 10.1186/s40478-022-01415-7. PMID: 35978418 PMCID: PMC9387077 DOI: 10.1186/s40478-022-01415-7
Acta Neuropathol Commun. 2022 Aug 17;10(1):114. doi: 10.1186/s40478-022-01415-7. PMID: 35978418 PMCID: PMC9387077 DOI: 10.1186/s40478-022-01415-7
Parkinson's Progression Markers Initiative brain autopsy program
Parkinsonism Relat Disord. 2022 Aug;101:62-65. doi: 10.1016/j.parkreldis.2022.06.017. Epub 2022 Jul 1. PMID: 35803091 DOI: 10.1016/j.parkreldis.2022.06.017
Parkinsonism Relat Disord. 2022 Aug;101:62-65. doi: 10.1016/j.parkreldis.2022.06.017. Epub 2022 Jul 1. PMID: 35803091 DOI: 10.1016/j.parkreldis.2022.06.017
