ADM - Advanced digital microscopy

Services

Services for IRB Barcelona and external researchers (public or private)

• Spectral laser scanning confocal microscopy. 3D, 4D, and 5D imaging with optical sectioning, custom spectral detection and resolution, high content or multiple position mode, and incubated environment control for live cells.
  Four LSM confocal units: inverted confocal systems (Leica SP5, LSM 780, LSM880) and one vertical confocal system (Leica SPE).
• Multiphoton microscopy. Deep tissue fluorescence imaging using multiphoton excitations and derived applications (SHG, etc…). Designed in the SP5 with a MAITAI ultrashort powder IR laser (710-990nm).
• Spinning disk confocal microscopy. Fast 5D imaging with the Andor Revolution system, equipped with EMCCD camera technology, FRAP photoactivation and microinjection.
• Multi-Mode Super Resolution System by SIM/ISM/SMLM: The Zeiss Elyra PS1 system combines confocal microscopy (LSM880) with three super resolution modalities: Image Scanning Microscopy (also known as Airyscan) , structured illumination microscopy (SIM), and single molecule localization microscopy (SMLM: STORM, PALM, and variants).
• High-content imaging/detectiont with automated wide-field fluorescence microscopy, confocal spinning disk, or TIRF:
  The Olympus ScanR enables a fully automated inverted microscope for wide imaging, multipouc and multiposition plates, automated live imaging with temperature incubation and CO2 control, super-stable illumination for high-quality, reproducible image analysis. content and total internal reflection (TIRF) microscopy for high-contrast images. on the surfaces.
  The Nikon LIPSI system enables fast, high-throughput screening imaging with the confocal spinning disk modality (Yokogawa CSU-W1) and multiple multi-well plate readings, thanks to the integrated multi-sample incubator that load samples automatically. The HTS can be performed on up to 20 plates. The system is also well suited for very long-term imaging (duration 1-2 weeks) as it allows robot-driven loading of multiple experiments in parallel.
• Laser manipulation of living cells and organisms. FRAP and laser nanosurgery combined in a wide-field fluorescence microscope to perform FRAP and fluorescence patterning, cell ablation, DNA damage, subcellular ablation, and correlative microscopy.
  The Zeiss Axiovert 200M laser cutter is a custom platform equipped with a pulsed UV laser (355nm, 470ps) and rotating disc detection.
• Light sheet microscopy for optically cleaned organs: a double-sided macroscope-based detection system and double-sided illumination for fluorescence-free 3D imaging and labeling of tissues/organs/organisms that they range from 0.5 mm to 2.5 cm.
• Optical Cleaning: We offer expertise with the latest optical tissue cleaning techniques such as BABB (Murray’s clear), CUBIC, DISCO family, ECI, DeepClear and more.
  Lightsheet Microscope based on LEGO bricks, LEMOLISH (http://legolish.org/lemolish) an open source instrument that allows lightsheet imaging of cleaned organs up to 5 cm in size.
• Light sheet microscopy for living organisms: a high-resolution double-sided illumination SPIM system, including multiple views and sample rotation, for full-volume live imaging, for samples ranging from 50 µm to 1 mm.
• Light Sheet-Based Oblique Plane Microscope: A light sheet-based system customized on an inverted microscope to enable imaging of multi-well plates and other conventional sample carriers similar to an plate.
• High Throughput OPM. A highly automated OPM system for high-content imaging of organoids in multiwell plates, part of the AECC-funded MACH3CANCER (https://mach3cancer.org) project.