Mutant mouse
GINYS-IRB-005
Dr. Stephen Forrow
Core Facility Manager
The facility was created in 2007. Our primary function is to generate genetically modified mouse models for users within IRB Barcelona. We are able to offer a comprehensive service for the generation of genetically altered mice including:
- Design of recombinant DNA molecules for genetic modification.
- Help with cloning protocols and reagents required to generate gene targeting and transgenic vectors.
- All cell-culture required for gene targeting in mouse embryonic stem (ES) cells, including expansion, transfection, drug selection, colony picking and colony expansion.
- Microinjection of DNA or ES cells into pre-implantation stage embryos.
- Electroporation of DNA/mRNA/proteins into pre-implantation stage embryos.
- Transfer of manipulated embryos into pseudo-pregnant foster mice.
- Subsequent processing of pups born to pseudopregnant fosters.
Services
The facility has a fully equipped laboratory for cell and molecular biology. Mouse embryonic stem (ES) cell culture is carried out within a dedicated cell culture laboratory.
We also have a small microinjection suite based within the PCB Animal Research Center (SEA). This suite contains inverted microscopes equipped with state of the art micromanipulators and microinjectors. The suite also contains everything necessary for mouse surgery and embryo manipulation.
We are equipped to carry out cryopreservation of mouse germ-line tissue.
Equipment
Molecular biology equipment
Cell biology Equipment
Embryo manipulation equipment
Publications
BMAL1-Driven Tissue Clocks Respond Independently to Light to Maintain Homeostasis
Cell 177, 1436–1447.e1–e12; May 30, 2019
Cell 177, 1436–1447.e1–e12; May 30, 2019
Differential requirements for Tousled-like kinases 1 and 2 in mammalian development
Cell Death And Differentiation 24 (11 ) 1872 - 1885 (2017)
Cell Death And Differentiation 24 (11 ) 1872 - 1885 (2017)
GEMC1 is a critical regulator of multiciliated cell differentiation
EMBO J . 2016 May 2;35(9):942-60
EMBO J . 2016 May 2;35(9):942-60
Hepatic overexpression of a constitutively active form of liver glycogen synthase improves glucose homeostasis
Journal Of Biological Chemistry 285 (48 ) 37170 - 37177 (2010)
Journal Of Biological Chemistry 285 (48 ) 37170 - 37177 (2010)