UAT - Genomics
Services
1) Real-time PCR (qPCR), with the following applications:
- Gene expression and transcriptional profile (absolute and relative quantification)
- MiRNA analysis (profiling, quantification) and non-coding RNA (nRNA) expression studies
- Analysis of genetic variation: detection of SNPs, CNV, genotyping of enzymes related to drug metabolism (DME)
- HRM (High Resolution Melt), for the detection of genetic variations
In addition to the use of self-service equipment (prior training of users), support is offered in experimental design and analysis of results. “Custom” expression experiments, including RNA extraction, experimental technique, and data analysis, can also be performed after consultation with UAT staff.
2) Quantification and quality control of nucleic acids using chip electrophoresis (Bioanalyzer):
- RNA quality analysis (RIN) and quantification
- Separation, size determination and quantification of DNA fragments
3) Quantification of nucleic acids using fluorimetric techniques (Picogreen ®, Ribogreen ®).
This quantification method is the most sensitive and specific, especially recommended when very precise quantification is required (e.g., to generate libraries for NGS), or when the concentration is very low (up to 25 pg / mL of dsDNA; up to a 1 ng). / ml RNA).
4)Sequencing by capillary electrophoresis, with the following applications:
- De novo sequencing, resequencing
- SNP detection, mutations and CNV analysis
- Confirmation of constructions obtained by cloning
- Microsatellite analysis
- Allelic discrimination
5) Authentication of human cell lines
It is a requirement increasingly demanded by many scientific journals. It is based on the analysis of STRs (9 polymorphism + amelogenin markers, which result in a unique profile for each cell line), according to ATCC recommendations.
Its applications are:
- Verification of the identity of established human cell lines.
- Detection of contamination with other cell lines, of the same species or of another species.
- Establishment of the profile of new cell lines (unique STR profile).
Equipment
- Real-time PCR:
- 7000 SDS (96p block) and 7900HT with Fast module (384p block and microfluidic cards) (Applied Biosystems)
- LightCycler480 (96p and 384p blocks) (Roche)
- Bioanalyzer 2100 (Agilent) . Electrophoresis system based on microfluidic chips (separation, sizing and automatic quantification of nucleic acid fragments).
- DNA1000 chip
- Nano and Pico RNA Chip
- Processing of any other type of chip provided by users.
- Capillary sequencing, in different formats (tubes and plates) and conditions. Free universal primers on demand. (*)
- Authentication of the human cell line. (*)
(*) Services provided through agreements with external suppliers.
Staff
