Single Cell

Equipment

10x Genomics Chromium™ System (10x genomics)

The unit is equipped with a Chromium Controller instrument. This technology is based on microfluidics and allows the generation in parallel of single cell or nuclei partitions with identifying barcodes and the preparation of sequencing libraries.

– Single cell transcriptome 3′ gene expression
– Chromium Single Cell ATAC (Assay for Transposase Accessible Chromatin)
– Chromium Single Cell Multiome ATAC + Gene Expression

Spatial Transcriptomics – Visium (10x genomics)

10x Genomics has developed a workflow for sequencing mRNA without the loss of spatial information. The Visium Spatial Gene Expression Solution allows for whole transcriptome and targeted analysis of mRNA using high-throughput sequencing, and subsequently maps a transcript’s expression pattern in tissue sections using high-resolution microscope imaging.

Tapestri Platform (Mission Bio)

The Tapestri Platform is designed for targeted single-cell sequencing. The platform is designed to produce targeted scDNA sequencing libraries alone or in a multi-omics solution which combines targeted single-cell DNA and protein analysis. Mission Bio offers the possibility of using pre-designed or custom DNA and protein panels.

-Tapestri Single-Cell targeted DNA Sequencing

-Tapestri Single-Cell targeted DNA + Protein Sequencing

Data Analysis

Single-cell and spatial genomics enable the comprehensive characterization of cells in their identity and function. However, single cell data are noisy and present several computational and analytical challenges, which require the application of tools specifically designed for their analysis. The unit will provide support for the bioinformatic analysis of data from individual cells.