New generation sequencing for HIV genotyping

Tests include genotypic antiretroviral resistance determination in reverse transcriptase, protease and integrase as well as genotypic HIV tropism testing. Resistance is usually determined from plasma, but can also be determined from PBMCs or low-viral- load plasma (ultracentrifugation) for patient with low viral loads or with non-evaluable results. The service, launched in 1999, was designated a reference centre for antiretroviral resistance testing by the Catalan Health Service in 2002. It also participates in research projects and clinical trials with other institutions and pharmaceutical compani


Sequencing Service


NSG Worflow
  • Sentosa SX101
  • Sentosa ST401i i ST401e
  • Sentosa SQ301
  • Sentosa SQ Reporter
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Responsable de Servei
Teresa Puig | | 934656374 ext 223 | ORCID | PRC Page
Tècnica de laboratori
Cristina Ramirez | | 934656374


Comparison of the LiPA HIV-1 RT test, selective PCR and direct solid phase sequencing for the detection of HIV-1 drug resistance mutations.
Schmit JC a, Ruiz L , Stuyver L , Van Laethem K , Vanderlinden I , Puig T , Rossau R , Desmyter J , De Clercq E , Clotet B , Vandamme AM
J Virol Methods . 1998 Jul;73(1):77-82. doi: 10.1016/s0166-0934(98)00043-3
Evolution of drug resistance in HIV-infected patients remaining on a virologically failing combination antiretroviral therapy regimen
Alessandro Cozzi-Lepri 1 , Andrew N Phillips, Lidia Ruiz, Bonaventura Clotet, Clive Loveday, Jesper Kjaer, Helene Mens, Nathan Clumeck, Ludmila Viksna, Francisco Antunes, Ladislav Machala, Jens D Lundgren, EuroSIDA Study Group
AIDS . 2007 Mar 30;21(6):721-32. doi: 10.1097/QAD.0b013e3280141fdf
Prevalence of transmitted antiretroviral resistance and distribution of HIV-1 subtypes among patients with recent infection in Catalonia (Spain) between 2003 and 2005.
Romero A, Sued O, Puig T, Esteve A, Pumarola T, Casabona J, González V, Matas L, Tural C, Rodrigo I, Margall N, Domingo P, Casanova A, Ferrer E, Caballero E, Ribera E, Farré J, Puig T, Amengual MJ, Navarro G, Prat JM, Masabeu A, Simó JM, Villaverde CA, Barrufet P, Sauca MG, Ortin X, Ortí A, Navarro R, Euras JM, Vilaró J, Villà MC, Montull S, Vilanova C, Pujol F, Díaz O, Miró JM; AERI study Group.
Enferm Infecc Microbiol Clin . 2011 Aug-Sep;29(7):482-9. doi: 10.1016/j.eimc.2011.03.001. Epub 2011 May 2
Prevalence of genotypic resistance to nucleoside analogues and protease inhibitors in Spain. The ERASE-2 Study Group
Puig T, Pérez-Olmeda M, Rubio A, Ruiz L, Briones C, Franco JM, Gómez-Cano M, Stuyver L, Zamora L, Alvarez C, Leal M, Clotet B, Soriano V.
AIDS. 2000 Apr 14;14(6):727-32. doi: 10.1097/00002030-200004140-00012. PMID: 10807196
Evaluation of the Innate Immune Modulator Acitretin as a Strategy To Clear the HIV Reservoir
Garcia-Vidal E, Castellví M, Pujantell M, Badia R, Jou A, Gomez L, Puig T, Clotet B, Ballana E, Riveira-Muñoz E, Esté JA.
Antimicrob Agents Chemother. 2017 Oct 24;61(11):e01368-17. Print 2017 Nov. PMID: 28874382
RNA editing by ADAR1 regulates innate and antiviral immune functions in primary macrophages
Pujantell M, Riveira-Muñoz E, Badia R, Castellví M, Garcia-Vidal E, Sirera G, Puig T, Ramirez C, Clotet B, Esté JA, Ballana E.
Sci Rep. 2017 Oct 17;7(1):13339. PMID: 29042669 Free PMC article
Long-term HIV-1 infection induces an antiviral state in primary macrophages
Pujantell M, Badia R, Ramirez C, Puig T, Clotet B, Ballana E, Esté JA, Riveira-Muñoz E.
Antiviral Res. 2016 Sep;133:145-55. Epub 2016 Aug 7. PMID: 27510577
The G1/S Specific Cyclin D2 Is a Regulator of HIV-1 Restriction in Non-proliferating Cells.
Badia R, Pujantell M, Riveira-Muñoz E, Puig T, Torres-Torronteras J, Martí R, Clotet B, Ampudia RM, Vives-Pi M, Esté JA, Ballana E.
PLoS Pathog. 2016 Aug 19;12(8):e1005829. eCollection 2016 Aug. PMID: 27541004 Free PMC article
Inhibition of herpes simplex virus type 1 by the CDK6 inhibitor PD-0332991 (palbociclib) through the control of SAMHD1.
Badia R, Angulo G, Riveira-Muñoz E, Pujantell M, Puig T, Ramirez C, Torres-Torronteras J, Martí R, Pauls E, Clotet B, Ballana E, Esté JA.
J Antimicrob Chemother. 2016 Feb;71(2):387-94. Epub 2015 Nov 4. PMID: 26542306 Free PMC article
RECall for automated genotypic tropism testing
Pou C, Bellido R, Casadellà M, Puig T, Clotet B, Harrigan R, Paredes R.
J Clin Microbiol. 2013 Aug;51(8):2754-7. Epub 2013 Jun 12. PMID: 23761150 Free PMC article.
Genotypic and phenotypic evolution of HIV type-1 protease during in vitro sequential or concomitant combination of atazanavir and amprenavir
Cunyat F, Ruiz L, Marfil S, Puig T, Bofill M, Blanco J, Clotet B, Cabrera C.
Antivir Ther. 2010;15(3):431-6. PMID: 20516562
Viral evolution during structured treatment interruptions in chronically human immunodeficiency virus-infected individuals
Martinez-Picado J, Frost SD, Izquierdo N, Morales-Lopetegi K, Marfil S, Puig T, Cabrera C, Clotet B, Ruiz L.
J Virol. 2002 Dec;76(23):12344-8. PMID: 12414975 Free PMC article.


IrsiCaixa - Institut de Recerca de la Sida

Campus Can Ruti
Hosp. Germans Trias i Pujol
Carretera Canyet, s/n

08916 Badalona


  • Education and knowledge generation system
  • Industrial system
  • Socio-sanitary system


    • Genomics
    • Other specializated laboratories
    • Sequencing


Availability Level: Medium
Access Procedures:
Open. Contact the person responsible for the service via email.


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Status: Active
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