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New generation sequencing for HIV genotyping

GINYS-IRSICAIXA-001
Teresa Puig
Responsable
tpuig@irsicaixa.es | 934656374 ext 223 | ORCID | PRC page
FACILITY WEBSITE
Tests include genotypic antiretroviral resistance determination in reverse transcriptase, protease and integrase as well as genotypic HIV tropism testing. Resistance is usually determined from plasma, but can also be determined from PBMCs or low-viral- load plasma (ultracentrifugation) for patient with low viral loads or with non-evaluable results. The service, launched in 1999, was designated a reference centre for antiretroviral resistance testing by the Catalan Health Service in 2002. It also participates in research projects and clinical trials with other institutions and pharmaceutical compani

Services

Sequencing Service

Equipment

NSG Worflow
  • Sentosa SX101
  • Sentosa ST401i i ST401e
  • Sentosa SQ301
  • Sentosa SQ Reporter
Miniplate spinner
Pipetes

Staff

Responsable de Servei
Teresa Puig | tpuig@irsicaixa.es | 934656374 ext 223 | ORCID | PRC Page
Tècnica de laboratori
Cristina Ramirez | cramirez@irsicaixa.es | 934656374

Publications

Comparison of the LiPA HIV-1 RT test, selective PCR and direct solid phase sequencing for the detection of HIV-1 drug resistance mutations.
Schmit JC a, Ruiz L , Stuyver L , Van Laethem K , Vanderlinden I , Puig T , Rossau R , Desmyter J , De Clercq E , Clotet B , Vandamme AM
J Virol Methods . 1998 Jul;73(1):77-82. doi: 10.1016/s0166-0934(98)00043-3
DOI: 0166-0934(98)00043-3
Evolution of drug resistance in HIV-infected patients remaining on a virologically failing combination antiretroviral therapy regimen
Alessandro Cozzi-Lepri 1 , Andrew N Phillips, Lidia Ruiz, Bonaventura Clotet, Clive Loveday, Jesper Kjaer, Helene Mens, Nathan Clumeck, Ludmila Viksna, Francisco Antunes, Ladislav Machala, Jens D Lundgren, EuroSIDA Study Group
AIDS . 2007 Mar 30;21(6):721-32. doi: 10.1097/QAD.0b013e3280141fdf
DOI: AD.0b013e3280141fdf
Prevalence of transmitted antiretroviral resistance and distribution of HIV-1 subtypes among patients with recent infection in Catalonia (Spain) between 2003 and 2005.
Romero A, Sued O, Puig T, Esteve A, Pumarola T, Casabona J, González V, Matas L, Tural C, Rodrigo I, Margall N, Domingo P, Casanova A, Ferrer E, Caballero E, Ribera E, Farré J, Puig T, Amengual MJ, Navarro G, Prat JM, Masabeu A, Simó JM, Villaverde CA, Barrufet P, Sauca MG, Ortin X, Ortí A, Navarro R, Euras JM, Vilaró J, Villà MC, Montull S, Vilanova C, Pujol F, Díaz O, Miró JM; AERI study Group.
Enferm Infecc Microbiol Clin . 2011 Aug-Sep;29(7):482-9. doi: 10.1016/j.eimc.2011.03.001. Epub 2011 May 2
DOI: .eimc.2011.03.001%20
Prevalence of genotypic resistance to nucleoside analogues and protease inhibitors in Spain. The ERASE-2 Study Group
Puig T, Pérez-Olmeda M, Rubio A, Ruiz L, Briones C, Franco JM, Gómez-Cano M, Stuyver L, Zamora L, Alvarez C, Leal M, Clotet B, Soriano V.
AIDS. 2000 Apr 14;14(6):727-32. doi: 10.1097/00002030-200004140-00012. PMID: 10807196
DOI: 0002030-200004140-00012%20
Evaluation of the Innate Immune Modulator Acitretin as a Strategy To Clear the HIV Reservoir
Garcia-Vidal E, Castellví M, Pujantell M, Badia R, Jou A, Gomez L, Puig T, Clotet B, Ballana E, Riveira-Muñoz E, Esté JA.
Antimicrob Agents Chemother. 2017 Oct 24;61(11):e01368-17. Print 2017 Nov. PMID: 28874382
DOI: 10.1128/AAC.01368-17
RNA editing by ADAR1 regulates innate and antiviral immune functions in primary macrophages
Pujantell M, Riveira-Muñoz E, Badia R, Castellví M, Garcia-Vidal E, Sirera G, Puig T, Ramirez C, Clotet B, Esté JA, Ballana E.
Sci Rep. 2017 Oct 17;7(1):13339. PMID: 29042669 Free PMC article
DOI: 10.1038/s41598-017-13580-0.%20
Long-term HIV-1 infection induces an antiviral state in primary macrophages
Pujantell M, Badia R, Ramirez C, Puig T, Clotet B, Ballana E, Esté JA, Riveira-Muñoz E.
Antiviral Res. 2016 Sep;133:145-55. Epub 2016 Aug 7. PMID: 27510577
DOI: 10.1016/j.antiviral.2016.08.004.
The G1/S Specific Cyclin D2 Is a Regulator of HIV-1 Restriction in Non-proliferating Cells.
Badia R, Pujantell M, Riveira-Muñoz E, Puig T, Torres-Torronteras J, Martí R, Clotet B, Ampudia RM, Vives-Pi M, Esté JA, Ballana E.
PLoS Pathog. 2016 Aug 19;12(8):e1005829. eCollection 2016 Aug. PMID: 27541004 Free PMC article
DOI: 10.1371/journal.ppat.1005829
Inhibition of herpes simplex virus type 1 by the CDK6 inhibitor PD-0332991 (palbociclib) through the control of SAMHD1.
Badia R, Angulo G, Riveira-Muñoz E, Pujantell M, Puig T, Ramirez C, Torres-Torronteras J, Martí R, Pauls E, Clotet B, Ballana E, Esté JA.
J Antimicrob Chemother. 2016 Feb;71(2):387-94. Epub 2015 Nov 4. PMID: 26542306 Free PMC article
DOI: 10.1093/jac/dkv363.%20
RECall for automated genotypic tropism testing
Pou C, Bellido R, Casadellà M, Puig T, Clotet B, Harrigan R, Paredes R.
J Clin Microbiol. 2013 Aug;51(8):2754-7. Epub 2013 Jun 12. PMID: 23761150 Free PMC article.
DOI: 10.1128/JCM.00935-13
Genotypic and phenotypic evolution of HIV type-1 protease during in vitro sequential or concomitant combination of atazanavir and amprenavir
Cunyat F, Ruiz L, Marfil S, Puig T, Bofill M, Blanco J, Clotet B, Cabrera C.
Antivir Ther. 2010;15(3):431-6. PMID: 20516562
DOI: 10.3851/IMP1543
Viral evolution during structured treatment interruptions in chronically human immunodeficiency virus-infected individuals
Martinez-Picado J, Frost SD, Izquierdo N, Morales-Lopetegi K, Marfil S, Puig T, Cabrera C, Clotet B, Ruiz L.
J Virol. 2002 Dec;76(23):12344-8. PMID: 12414975 Free PMC article.
DOI: 10.1128/jvi.76.23.12344-12348.2002

INSTITUTE

IrsiCaixa - Institut de Recerca de la Sida

Campus Can Ruti
Hosp. Germans Trias i Pujol
Carretera Canyet, s/n

08916 Badalona http://www.irsicaixa.es/es

DOMAINS RIS3CAT

  • Education and knowledge generation system
  • Industrial system
  • Socio-sanitary system

CATEGORIES

  • CLINICAL TRIALS
  • TECHNOLOGICAL EQUIPMENT
    • Genomics
    • Other specializated laboratories
    • Sequencing

RATES AND ACCESS

Tarifes_GINYS-IRSICAIXA-001_1-2.pdf
Availability Level: Medium
Access Procedures:
Open. Contact the person responsible for the service via email.

QUALITY CONTROL

HRS4R
Human Resource Strategy for Researchers It supports research institutions and funding organizations in implementing the Charter and Code in their policies and practices. The implementation of the Charter and Code principles by research institutions makes them more attractive to researchers looking for a new employer or host for their research project..
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Status: Active
CERCA - Ginys