Nuclear magnetic resonance
GINYS-ICIQ-001
The Nuclear Magnetic Resonance (NMR) Unit provides researchers with access to a wide range of NMR experiments both under automation or manual mode.
It also advises researchers on the most appropriate experiment to their needs and it trains and instructs researchers in using NMR, what information can be obtained from NMR experiments and how to optimize the instrumental resources available.
The techniques available up to now guarantee access to all kinds of NMR experiments in liquid phase for a wide range of nuclei and in gel phase for the more common nuclei (1H and 13C). The NMR unit has successfully introduced researchers to such uncommon techniques as heteronuclear (mono and bidimensional) experiments, diffusion experiments and kinetic measurements.
The unit staff also organizes training activities which range from training new users in obtaining NMR spectra automatically and how to use NMR processing software (TOPSPIN), to more general courses about the NMR experiments available and the information they can provide.
Services
1) MONODIMENSIONAL
Standard analysis of small molecules
-) Standard 1H (proton) experiments
-) Standard 13C{1H} (carbon with
proton decoupling) experiments
proton decoupling) experiments
-) DEPT/DEPTQ experiments. Carbon experiments with carbon
signals separated according to multiplicity.
signals separated according to multiplicity.
-) 1H and 13C{1H} qNMR
(quantitative) experiments.
(quantitative) experiments.
Experiments performed in anyone of the available instruments
(300 MHz, 400 MHz or 500 MHz)
(300 MHz, 400 MHz or 500 MHz)
2) BIDIMENSIONAL
Analysis of medium sized molecules. Molecular mapping
-) NOESY and ROESY experiments. 1H spatial
orientation.
orientation.
-) HMQC and HSQC experiments. 1H – 13C
one-bond connectivity.
one-bond connectivity.
-) HMBC experiments. 1H – 13C
several-bond connectivity.
several-bond connectivity.
-) HOESY experiments. 1H versus 13C
spatial orientations.
spatial orientations.
-) INADEQUATE experiments. 13C – 13C
connectivity.
connectivity.
Experiments performed in anyone of the available instruments
(300 MHz, 400 MHz or 500 MHz) or in the 500 MHz instruments equipped with a
cryoprobe (depending on the experiment/sample concentration).
(300 MHz, 400 MHz or 500 MHz) or in the 500 MHz instruments equipped with a
cryoprobe (depending on the experiment/sample concentration).
3) MULTINUCLEAR
Analysis of complex molecules with heteroatoms.
-) Direct acquisition of less common nuclei experiments (31P, 19F, 15N, 11B,
29Si, 195Pt, 119Sn).
29Si, 195Pt, 119Sn).
-) COSY and TOCSY experiments. X – X connectivity (X may be 31P,
19F).
19F).
-) HMQC, HSQC and HMBC experiments. 1H – X
connectivity (X may be 31P, 19F, 15N, 11B,
29Si, 195Pt, 119Sn).
connectivity (X may be 31P, 19F, 15N, 11B,
29Si, 195Pt, 119Sn).
-) HMQC, HSQC and HMBC experiments. 19F – X
connectivity (X may be 13C, 31P, 15N, 11B,
29Si, 195Pt, 119Sn).
connectivity (X may be 13C, 31P, 15N, 11B,
29Si, 195Pt, 119Sn).
-) HMQC, HSQC and HMBC experiments. 31P
– X connectivity (X may be 13C, 15N, 11B, 29Si,
195Pt, 119Sn).
– X connectivity (X may be 13C, 15N, 11B, 29Si,
195Pt, 119Sn).
-) 13C{1HX}
experiments. 13C experiments with double decoupling, proton and X (31P
or 19F).
experiments. 13C experiments with double decoupling, proton and X (31P
or 19F).
-) 31P{1H19F}
experiments. 31P experiments with double decoupling, proton and fluorine.
experiments. 31P experiments with double decoupling, proton and fluorine.
Experiments
performed either on the 500 MHz instrument equipped with three channels or in
the 500 MHz instrument equipped with a cryoprobe.
performed either on the 500 MHz instrument equipped with three channels or in
the 500 MHz instrument equipped with a cryoprobe.
4) VARIABLE TEMPERATURE/DIFFUSION
EXPERIMENTS
EXPERIMENTS
Study of dynamic/complex systems
-) Variable temperature (heating and/or cooling)
experiments, on all the available nuclei. From -150 C to + 150 C (if the
solvent allows it)
experiments, on all the available nuclei. From -150 C to + 150 C (if the
solvent allows it)
-) DOSY (diffusion) experiments on all the available nuclei.
Determination of number of species present/diffusion coefficients of molecules.
Determination of number of species present/diffusion coefficients of molecules.
Experiments performed either on the 500 MHz instrument
equipped with three channels or in the 500 MHz instrument equipped with a
cryoprobe.
equipped with three channels or in the 500 MHz instrument equipped with a
cryoprobe.
Equipment
- Ultrashield Bruker Avance NEO 300 NMR Spectrometer
- Bruker Avance 400 Ultrashield NMR Spectrometer
- Ultrashield Bruker Avance NEO 500 NMR Spectrometer
- Ultrashield Bruker Avance II 400 NMR Spectrometer
- Bruker Avance III 500 Ultrashield Plus NMR Spectrometer
Staff
Israel Macho | imacho@iciq.es
| 977 920 200 (ext. 310)
Dr. Gabriel González | ggonzalez@iciq.es
| 977 920 200 (ext. 310)
