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Proteomics

GINYS-CRG-004
Eduard Sabidó
Head of Unit
eduard.sabido@crg.eu | ORCID | PRC page
FACILITY WEBSITE
Carrer Doctor Aiguader 88
08003 Barcelona
Spain
The Proteomics Unit is a joint effort of Pompeu Fabra University and the Center for Genomic Regulation to create an innovative research infrastructure with a high level of excellence and sustainability to support scientists in the biomedical field. As part of the Spanish National Omics Infrastructure ICTS OmicsTech (www.omicstech.es), the Proteomics Unit is a national reference unit for proteomics analyses.

The Unit's activities focus on providing high-quality proteomics services with strong added value to the research community through:

  •      Advice, expertise and experience in proteomics analyses
  •      Development of new methods and facilitating techniques for cutting-edge research and innovation
  •      Training and the active dissemination of new methods and scientific advances in the field of proteomics

Services

Characterization of proteome interactions

  • Identification of protein-protein interactions
  • Identification of protein-DNA interactions
  • Identification of protein-RNA interactions

Characterization of post-translational modifications

  • Identification and quantification of acetylations, methylations, and ubiquinations in purified proteins
  • Identification and quantification of post-translational modifications in purified histones.
  • Identification and quantification of phosphopeptides

Characterization of complete proteomes

  • Proteome quantification (with and without label – TMT, SILAC, etc.)
  • Phosphoproteome quantification (with and without label – TMT, SILAC, etc.)
  • Proteome characterization of liquid biopsies (plasma, cerebrospinal fluid)

Analysis of a single protein

  • Confirmation of overexpressed proteins in complex samples
  • Identification of a protein in gel bands
  • Molecular Weigh determination of intact proteins

Targeted proteomics

  • Peptide selection for biomarker quantification using targeted proteomics
  • Optimization of targeted proteomics methods for biomarker quantification
  • Quantification of biomarkers using targeted proteomics

RNA and DNA modifications

  • Analysis of modified (ribo-)nucleosides by mass spectrometry

Equipment

As a reference proteomics center, the Proteomics Unit has state-of-the-art equipment including high-performance liquid chromatography systems and high-resolution Orbitrap mass spectrometers to identify and quantify proteins of interest.

The instruments available in the Proteomics Unit cover the most advanced techniques in the field of proteomics, and are accessible to national and international scientists.

Staff

Eduard Sabidó | eduard.sabido@crg.eu | ORCID | PRC Page

Projects

Title: EU-LIFE: Alliance of research institutes advocating for excellent research in Europe
Reference: https://eu-life.eu/
Funding Organism: EU
Status: Executing
Title: EPIC-XS: Proteomics (2019-2022)
Reference: https://epic-xs.eu/
Funding Organism: EU
Status: Executing

Publications

BRCA1 mutations in high-grade serous ovarian cancer are associated with proteomic changes in DNA repair, splicing, transcription regulation and signaling
Melissa Bradbury, Eva Borràs, Josep Castellví, Olga Méndez, José Luis Sánchez-Iglesias, Assumpció Pérez-Benavente, Antonio Gil-Moreno, Eduard Sabidó & Anna Santamaria
Scientific Reports. 12 (1): 4445- - 2022-03-15 12(1)
DOI: 10.1038/s41598-022-08461-0
Urinary metabolic biomarkers of diet quality in European children are associated with metabolic health
Nikos Stratakis, Alexandros P Siskos, Eleni Papadopoulou, Anh N Nguyen, Yinqi Zhao, Katerina Margetaki, Chung-Ho E Lau, Muireann Coen, Lea Maitre, Silvia Fernández-Barrés, Lydiane Agier, Sandra Andrusaityte, Xavier Basagaña, Anne Lise Brantsaeter, Maribel Casas, Serena Fossati, Regina Grazuleviciene, Barbara Heude, Rosemary RC McEachan, Helle Margrete Meltzer, Christopher Millett, Fernanda Rauber, Oliver Robinson, Theano Roumeliotaki, Eva Borras, Eduard Sabidó, Jose Urquiza, Marina Vafeiadi, Paolo Vineis, Trudy Voortman, John Wright, David V Conti, Martine Vrijheid, Hector C Keun, Leda Chatzi
Elife. 11 (e71332): - 2022-01-25 11(e71332),
DOI: 10.7554/eLife.71332
Molecular characterization of triple negative breast cancer formaldehyde-fixed paraffin-embedded samples by data-independent acquisition proteomics
Silvia García-Adrián,Lucía Trilla-Fuertes,Angelo Gámez-Pozo,Cristina Chiva,Rocío López-Vacas,Elena López-Camacho,Andrea Zapater-Moros,María I. Lumbreras-Herrera,David Hardisson,Laura Yébenes,Pilar Zamora,Eduard Sabidó,Juan Ángel Fresno Vara,Enrique Espinosa
Proteomics. 22 (3): - 2022-01-01 22(3),
DOI: 10.1002/pmic.202100110
Multi-laboratory experiment PME11 for the standardization of phosphoproteome analysis
Núria Colomé, Joaquín Abian, Kerman Aloria, Jesús M. Arizmendi, Silvia Barceló-Batllori, Sophie Braga-Lagache, Odile Burlet-Schiltz, Montse Carrascal, J. Ignacio Casal, Eduard Chicano-Gálvez, Cristina Chiva, Luis Felipe Clemente, Felix Elortza, Josep M. Estanyol, Joaquín Fernandez-Irigoyen, Patricia Fernández-Puente, María José Fidalgo, Carine Froment, Manuel Fuentes, Carlos Fuentes-Almagro, Marina Gay, Alexandre Hainard, Manfred Heller, María Luisa Hernández, Nieves Ibarrola, Ibon Iloro, Thomas Kieselbach, Antonio Lario, Marie Locard-Paulet, Anabel Marina-Ramírez, Luna Martín, Esperanza Morato-López, Javier Muñoz, Rosana Navajas, M. Antonia Odena, Leticia Odriozola, Eliandre de Oliveira, Alberto Paradela, Carla Pasquarello, Vivian de los Rios, Cristina Ruiz-Romero, Eduard Sabidó, Manuel Sánchez del Pino, Jaime Sancho, Enrique Santamaría, Christine Schaeffer-Reiss, Justine Schneider, Carolina de la Torre, M. Luz Valero, Marta Vilaseca, Shuai Wu, Linfeng Wu, Pilar Ximénez de Embún, Francesc Canals, Fernando J. Corrales,
Journal Of Proteomics. 251 - 2022-01-01 251(),
DOI: 10.1016/j.jprot.2021.104409
High-performance nano-flow liquid chromatography column combined with high- and low-collision energy data-independent acquisition enables targeted and discovery identification of modified ribonucleotides by mass spectrometry
Guadalupe Espadas, Julia Morales-Sanfrutos, Rebeca Medina, Morghan C Lucas, Eva Maria Novoa, Eduard Sabidó,
Journal Of Chromatography a. 1665 - 2022-01-01 1665(),
DOI: 10.1016/j.chroma.2022.462803

INSTITUTE

CRG - Centre de Regulació Genòmica

Parc de Recerca Biomèdica de Barcelona (PRBB)
Dr. Aiguader, 88

08003 Barcelona http://www.crg.eu/

SHARED WITH

DOMAINS RIS3CAT

  • Education and knowledge generation system
  • Industrial system
  • Socio-sanitary system

CATEGORIES

  • TECHNOLOGICAL EQUIPMENT
    • Proteomics
    • Spectrometry, chromatography and spectroscopy

RATES AND ACCESS

CRG-proteomics_2022r1
Availability Level: High
Access Procedures:
Open. Procedure

FACILITIES NETWORKS

PROTrEIN – Training of computational proteomics researchers
European network of innovative and interdisciplinary research and training to train the next generation of researchers in computational proteomics. The network brings together the best international experts in computational proteomics, network biology, data visualization, mass spectrometry and gamification from prestigious European academic and private institutions and is made up of 11 beneficiaries and 8 associated organizations
Network link
Reach: Europe
Status: Active
PRC page
ICTS OmicsTech – ICTS OmicsTech node
Mission: Realitzar estudis d'anàlisi de genoma, proteoma i metaboloma que permetin millorar significativament la salut i la qualitat de vida de les persones, en col·laboració amb les comunitats de recerca catalana, espanyola, europea i internacional. To promote the Omics Sciences in Spain, satisfying the growing demand for sequencing, proteome and metabolome analysis and ensuring the competitiveness of Spain in technological applications in the fields of Biomedicine, Biology and the Agri-Food sector.
Network link
Reach: Spain
Status: Active
PRC page
PRB3-PROTEORED – Biomolecular Resources Platform: PROTEORED Proteomic Platform
Contribute to the design and development of projects in the field of biotechnology and biomedicine, establish new concepts in biology, identify new diagnostic and therapeutic targets, promote the development of new biopharmaceuticals and dissect the mechanism of action. of drugs and their selective effects on specific individuals.
Network link
Reach: Spain
Status: Active
PRC page
ProMed – Proteomic Platform
The Center for Genomic Regulation (CRG) has partnered with several leading hospital research institutes in Catalonia to create a new state-of-the-art infrastructure to study and analyze proteins. It is the first such collaboration between biomedical research institutes and hospitals. The state-of-the-art equipment acquired will help meet the growing demand for protein analysis from Catalan research and innovation institutions.
Network link
Reach: Catalonia
Status: Active
PRC page
CERCA - Ginys