Protein technologies
GINYS-CRG-003
The Biomolecular and Protein Screening Technologies Unit (BMS & PT) offers CRG-CNAG / PRBB researchers, external institutions and private companies a new high-quality technological platform to carry out the production of proteins and automated processes in the context of biomolecular and biochemical screening assays.
Through staff-assisted detection, users will have access to facility equipment, including liquid handlers, plate readers, and small molecule libraries.
In addition, the unit aims to support CRG institute researchers by purchasing, researching, and maintaining valuable stocks of clones and strains.
Services
- Full service production of plasmid clones, mutants and plasmid libraries.
- High performance cloning
- Production of recombinant proteins and protein complexes: bacteria, insect cells and mammalian expression
- Purification of proteins and antibodies
- Biophysical characterization of proteins and nucleic acids:
- Surface plasmon resonance, microscale thermophoresis, size exclusion chromatography – multiangle light scattering
- Automation of biomolecular and biochemical screening assays
- Genome editing using the Crispr-Cas9 system: knockout / Knockin gene cell line engineering in collaboration with the CRG Tissue Engineering Unit
Equipment
- ÄKTA Xpress twin and ÄktaFPLC system for protein purification (GE Healthcare)
- Biacore T100 (SPR)
- Monolith NT.115 Thermophoresis (MST)
- UPLC (shimatzu) – DAWN8 + Optilab® T-rEX ™
- LabCHIP GXII
- TECAN Freedom evo 200 robotic platform
- Caliper Zephyr robotic platform
- Caliper Sciclone robotic platform
- Biotek ELx405 washing machine
- Kendro incubator
- MultidropV2
- Tecan M200 plate reader
- Electroporator Amaxa 96-inch plate
siRNA libraries
The entire human genome collection siGENOME SMARTPoool, (Dharmacon, Thermo-Fisher) consists of groups of 4 different siRNAs per gene and is distributed in four “sets” as follows
- Kinases – 779 gens
- GPCR – 516 people
- “Drugable” genome: 6022 genes
- Remaining genome collection: 13803 genes
Chemical compound libraries
- The Prestwick Chemical Library®: 1200 small molecules, 100% marketed drugs
- Prestwick Phytochemical Library – 320 natural products
- Tocris kinase inhibitor toolbox (Tocris): 80 small molecule compounds
Staff
Projects
Title:
ARBRE: The association for resources for biophysical research in Europe
Reference:
https://arbre-mobieu.eu/
Funding Organism:
COST-UE
Status:
Executing
Title:
EPIC-XS: Proteomics (2019-2022)
Reference:
https://epic-xs.eu/
Funding Organism:
EU
Status:
Executing
Publications
Multiplex Antibody Analysis of IgM, IgA and IgG to SARS-CoV-2 in Saliva and Serum From Infected Children and Their Close Contacts
Frontiers In Immunology. 13 (751705): 751705-751705 - 2022-01-27 13(751705),
Frontiers In Immunology. 13 (751705): 751705-751705 - 2022-01-27 13(751705),
Determinants of early antibody responses to COVID-19 mRNA vaccines in a cohort of exposed and naïve healthcare workers
Ebiomedicine. 75 - 2022-01-01 75()
Ebiomedicine. 75 - 2022-01-01 75()
Matrix-screening reveals a vast potential for direct protein-protein interactions among RNA binding proteins
Nucleic Acids Research. 49 (12): 6702-6721 - 2021-07-09 49(12)
Nucleic Acids Research. 49 (12): 6702-6721 - 2021-07-09 49(12)
Immunogenicity and crossreactivity of antibodies to the nucleocapsid protein of SARS-CoV-2: utility and limitations in seroprevalence and immunity studies
Transl Res. 23260-74 - 2021-06-01 232()
Transl Res. 23260-74 - 2021-06-01 232()
A multiplex antigen microarray for simultaneous IgG and IgM detection against SARS-CoV-2 reveals higher seroprevalence than reported
Microbial Biotechnology. 14 (3): 1228-1236 - 2021-05-01 14(3)
Microbial Biotechnology. 14 (3): 1228-1236 - 2021-05-01 14(3)
