Histology platform
GINYS-IDIBELL-002
The Histology Platform provides our users with the appropriate infrastructures to carry out the most common histological techniques, such as inclusions, microtomies, colorimetric stains and immunodetections (direct or indirect), as well as to develop other newer or more complex techniques.
In addition, it offers the processing of varied samples: cells and colonies grown in monolayer and three-dimensional structures such as embryonic bodies and organoids, obtained in vitro and thin sections of tissues / organs on slides (paraffin sections or cryosections, 3-10 µm) and thick tissue sections to be processed on floating or in toto (30-300µm).
The platform allows service requests to be made with technical support, in self-service or in a combined mode, and supports both campus and external users.
In addition, it also participates in training tasks, with trainees both from CFGS and university level, and with courses, seminars and workshops.
Services
- Manual and automatic inclusions (in paraffin and OCT / Tissue tek)
- Microtomy, cryomicrotomy and vibratomy
- Histological stains (H&E, Masson’s Trichrome, Safranin-Fast Green, May Grünwald-Giemsa…)
- Immunocytochemistry and immunohistochemistry with Fluorescence and HRP / DAB
- Enzymatic techniques for the detection of cell proliferation (BrdU and EdU kits) and apoptosis (TUNEL)
Equipment
- 1 Tissue processor/robot – Leica TP1020
- 1 Paraffin Station – Leica EG1150H
- 2 Cold plate – Leica EG1150C
- 2 Rotary microtome – Leica RM2855 / Thermo Fisher Microm HM 340 E
- 2 Flotation bath – Leica HI1210
- 1 Autostainer – Leica XL
- 1 Autostainer – Dako Plus
- 2 Cryostat – Leica CM3050S
- 1 Vibratome – Leica VT1000S
- 1 Declaoking chamber – Biocare Medical NxGene
- 1 Sliding microtome – Leica SM2000R
- 1 Hibridization stove – Thermo Electron HBSNSRS220
- 2 Stoves – Selecta 2000692 / Selecta 2000207
- 1 Optical microscope – Leica DM1000
- 2 Steromicroscope – Leica S6E + Light source Leica L2 / Olympus SZX2-ILLB
- 1 Fume hood – Burdinola B ST 1500 LOW
Staff
Publications
Turning human epidermis into pancreatic endorderm
Rev Diabet Stud. 7(2): 158-167. 10 August 2010
Rev Diabet Stud. 7(2): 158-167. 10 August 2010
Characterization of pluripotent stem cells
Nature Protocols vol. 8, pages 223–253. 10 January 2013;
Nature Protocols vol. 8, pages 223–253. 10 January 2013;
Mcad-mediated intercellular interactions activate satellite cell division
J Cell Sci 126 (Pt 22):5116-31. Epub 2013 Sep 17.
J Cell Sci 126 (Pt 22):5116-31. Epub 2013 Sep 17.
The local microenvironment limits the regenerative potential of the mouse neonatal heart
Science Advances vol. 4, no. 5. 2 May 2018
Science Advances vol. 4, no. 5. 2 May 2018
