Molecular interaction platform
GINYS-IDIBELL-006
The Molecular Interactions Platform provides scientific-technological support to the scientific community in the field of molecular interactions and metabolism using several approaches. First, Surface Plasmon Resonance (SPR) allows real-time monitoring of any type of molecular interactions (protein-protein, protein-drugs, protein-vesicles, protein-RNA, …) and performs high-precision measurements of affinity and kinetic constants, binding specificity, isotyping and screening of potential medications/drugs. Next, Luminex technology allows the detection of up to fifty different biomarkers at once in biological samples and fluids using the technology of magnetic beads. Finally, metabolism and bioenergetics, driving cellular functions such as cell signalling, proliferation, or cell fate, can be measured with the Seahorse in real-time in living cells. Mitochondrial respiration, glycolysis, ATP rate oxidation and fatty acid oxidation can be measured with high precision.
Our mission is to provide an integrated support, from experimental design, optimization of assay conditions, experiment execution to data analysis and reporting. Our state-of-the art technology together with the large experience and know-how in biomedical research and protein science are at the service of both internal and external researchers.
Services
- Determination of affinity constant (KD), kinetics: association and dissociation constants (kon, koff) in different models (protein-protein, protein-peptide, protein-small molecule, protein-RNA …)
- Determination of effective concentration and quality control
- Antibody isotyping
- Screening of potential drugs/biosimilars
- Quantification of up to 50 biomarkers in biological fluids and samples in 96-well plate by Luminex
- Measure of oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) in 96-well plate (Seahorse): mitohocondrial respiration, glycolysis, ATP production, lipolysis
- Fluorescent Scanning of several formats
- Set up and optimization of experimental conditions
- Scientific-technological support to researchers
- Training of new users in using the equipement
Equipment
- Biacore T-200 (GE) for SPR analysis of interactions
- Seahorse XFe96 Analyzer (Agilent) for detection of OCR and ECAR in 96-well plate
- Magpix (Luminex) including xPonent 4.2 software for multiplex analysis of up to 50 magnetic beads
- Fluorescence scanner Typhoon FLA9500 (GE) equipped with blue, green and red laser
- ImageQuant TL 7.0 software
Staff
Silvia Barceló | molecularinteractions@idibell.cat
Publications
SIRT6-dependent cysteine monoubiquitination in the PRE-SET domain of Suv39h1 regulates the NF-kB pathway.
Nature communications, 2018, 9:101
Nature communications, 2018, 9:101
KAT6B is a tumor suppressor histone H3 lysine 23 acetyltransferase undergoing genomic loss in small cell lung cancer.
Cancer Research 2015, 75, 3936-3945
Cancer Research 2015, 75, 3936-3945
Integration of DIGE and bioinformatics analyses reveals a role of the anti-obesity agent tungstate in redox and energy homeostasis pathways in brown adipose tissue.
Molecular and Cellular Proteomics, 2008, 7, 378-393
Molecular and Cellular Proteomics, 2008, 7, 378-393