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Microscopy platform

GINYS-IRSP-001
Esther Peña Sendra
Core Facility Manager
microscopia@santpau.cat | 93 553 7892 | ORCID | PRC page
FACILITY WEBSITE
Institut de Recerca de l’Hospital de la Santa Creu i Sant Pau Centre CERCA
Institut d’Investigació Biomèdica Sant Pau (IIB Sant Pau)
Sant Quintí , 77-79 
Barcelona – 08041
The Confocal Microscopy Platform is a platform to support biomedical research. Its main function is to advise on experimental design, choice of equipment according to needs and training in the use, acquisition, quantification, and analysis of images.

Services

  • Advice on the preparation and / or approach of studies.
  • Training.
  • Immunofluorescence of 2 to 5 fluorochromes.
  • 3D and 4D image reconstruction.
  • Colocation studies.
  • Inter and intra-interactions (FRET and FLIM).
  • Conventional fluorescence or clear field microscopy in living, fixed, and tissue cells.
  • Time lapse
  • Anatomopathological studies, of different tissues, stained by immunohistochemistry (freezing, or paraffin) and quantification of images.

Equipment

  • Leica SP5 AOBS multispectral confocal with high-resolution high-speed tandem scanning and scanning system, FLIM / FCS modules and time-lapse incubation system.
  • Leica SP2 AOBS multispectral confocal with high-resolution high-speed tandem scanning and scanning system, and time-lapse incubation system
  • Zeiss AXIOVERT 200M inverted fluorescence microscope with Tª and CO2 control, with incubation cabin that allows you to work with living cells with both fluorescence and clear field. Equipped with fluorescence filters: DAPI, FITC, Rhodamine. Equipped with polarizers that allow the capture of images of polarized light
  • Olympus BX51 microscope with fluorescence with double head. Equipped with fluorescence filters: DAPI, FITC, TRITC, TXRED.
  • Nikon Eclipse 80i microscopes. Equipped with fluorescence filters: FITC, G-1B (546), FITC-CY3.
  • Stand for macro photographs with lighting
  • LEICA MZ 9.5 stereomicroscopes

Staff

Core Facility Manager
Esther Peña Sendra | EPena@santpau.cat | ORCID | PRC Page
Senior Technician
J. Oriol Juan Babot | OJuan@santpau.cat | +34 935565889 | ORCID
Esther Peña Sendra | EPena@santpau.cat | ORCID | PRC Page

Publications

Endothelium-Released Microvesicles Transport miR-126 That Induces Proangiogenic Reprogramming in Monocytes.
Arderiu G, Peña E, Civit-Urgell A, Badimon L.
Front Immunol. 2022 Feb 18;13:836662.
DOI: immu.2022.836662.%20eCollection%202022
mCRP triggers angiogenesis by inducing F3 transcription and TF signalling in microvascular endothelial cells.
Peña E, de la Torre R, Arderiu G, Slevin M, Badimon L.
Thromb Haemost. 2017 Jan 26;117(2):357-370.
DOI: H16-07-0524.

INSTITUTE

IRSantPau - Institut de Recerca Sant Pau

Sant Quintí, 77-79

08041 Barcelona http://www.recercasantpau.cat/

DOMAINS RIS3CAT

  • Education and knowledge generation system
  • Industrial system
  • Socio-sanitary system

CATEGORIES

  • TECHNOLOGICAL EQUIPMENT
    • Biology Laboratory
    • Microscopy

RATES AND ACCESS

Availability Level: High
Access Procedures:
Open. See website for conditions

QUALITY CONTROL

ISO 9001 – Quality Management
ISO 9001 sets out the criteria for a quality management system. This standard is based on a number of quality management principles that include a strong customer focus, motivation and involvement of senior management, process focus, and continuous improvement. The use of the ISO 9001 standard helps to ensure that customers receive consistent and good quality products and services, which in turn brings many business benefits.
Website link
Status: Active
CERCA - Ginys