Genomics and transcriptomics platform
GINYS-IRSP-005
The Genomics and Transcriptomics platform is a core facility focused on giving advice, offering specific services in genomics or transcriptomics techniques, and making specific equipment available to researchers and the scientific community.
Services
Specifically, GT Platform offers the following services:
- Quantitation and quality control of nucleic acid samples (Qubit 3.0, Nanodrop 2000, and Bioanalyzer 2100)
- Sample processing for Microarrays studies (Gene expression, miRNA profiles, Cytogenetics, GWAS)
- Real-time PCR projects (Gene expression, miRNA detection, allelic discrimination, CNV, absolute quantification….)
- Next-Generation Sequencing: exome sequencing, targeted RNA or DNA sequencing (Ampli-Seq technology), small RNA sequencing, de novo sequencing, ChiP-Seq, 16S sequencing, metagenomics…
- Sanger Sequencing: DNA sequencing, mutation and polymorphism analysis, DNA methylation studies, microorganisms identification…
- Fragment analysis: cell line authentication, copy number (MLPA), LOH, genotyping studies, microsatellites instability, DNA repeat expansion studies….
- Omics Data analysis
Some of the equipment used to carry out these techniques can be accessible for researchers after specific user training.
Equipment
Specific Equipment
- Affymetrix GS 3000 System array platform (upgrade 7G).
- 7900HT Fast Real-Time PCR system (96-well, 384-well, and TLDA blocs) (AB-ThermoFisher Sci)
- Bioanalyzer 2100 (Agilent Tech).
- Nanodrop 2000 (ThermoFisher Sci).
- Qubit 3.0 (ThermoFisher Sci)
- Infinite M200 PRO (Tecan) microplate reader: spectrophotometer, fluorimeter, luminometer.
- 3130xl Genetic Analyzer capillary electrophoresis sequencer (ThermoFisher Sci).
- Ion GeneStudio S5 Next-Generation Sequencing System, with Ion Chef System (ThermoFisher Sci).
Staff
