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Cell culture platform

GINYS-IRSP-006
Estefania Segales
Coordinació
ESegales@santpau.cat |
FACILITY WEBSITE
Currently, in vitro cell techniques are a fundamental tool for research carried out by a wide array of scientific groups. The IR Cell Culture Service offers a suitable infrastructure for producing primary cultures and cell lines at different biosafety levels (BSL2 or BSL3), as well as bacterial cultures at biosafety level I.

Equipment

The service consists of different facilities where you can work with both primary cultures obtained from human tissues and experimental animals, as well as continuous cell lines and Genetically Modified Organisms (GMOs) level I and II. In each of the facilities users have the appropriate and basic equipment needed to obtain and maintain cell cultures: laminar flow booths and biological safety booths (a total of 25), CO2 incubators (28) , stoves, centrifuges, water baths, inverted microscopes, scales, vacuum traps, cell counters, refrigerators, freezers (-20 and -80 ° C), hybridization ovens and autoclaves.

These facilities include equipment such as: special biosafety booths to work with cytostatic and / or nanoparticle products, microscopes with fluorescence and image capture, binocular microscopes with lighting and display screen, particle counter , UV stations, nucleofection equipment.

In addition, the service has hypoxia stations, a laboratory restricted by the use of radioactivity in crops, a laboratory to carry out bacterial cultures and a laboratory where biological samples can be processed (unrelated with the obtaining of crops) that require manipulation in conditions of biosecurity.

Staff

Estefania Segales | ESegales@santpau.cat

INSTITUTE

IRSantPau - Institut de Recerca Sant Pau

Sant Quintí, 77-79

08041 Barcelona http://www.recercasantpau.cat/

DOMAINS RIS3CAT

  • Food system
  • Education and knowledge generation system
  • Industrial system
  • Socio-sanitary system

CATEGORIES

  • TECHNOLOGICAL EQUIPMENT
    • Histology

RATES AND ACCESS

Availability Level: Medium
Access Procedures:

Open

QUALITY CONTROL

ISO 9001 – Quality Management
ISO 9001 sets out the criteria for a quality management system. This standard is based on a number of quality management principles that include a strong customer focus, motivation and involvement of senior management, process focus, and continuous improvement. The use of the ISO 9001 standard helps to ensure that customers receive consistent and good quality products and services, which in turn brings many business benefits.
Website link
Status: Active
Biosafety Level 3 (or NBS3)
It is already considered a high level of containment, it is found in some clinical and diagnostic laboratories, and some university and government research laboratories, in which work is done with exotic agents or that can cause serious damage and potentially fatal as a result of inhalation or exposure to them (some haemorrhagic fever viruses, but not filoviruses, many arboviruses and bacteria with Francisella tularensis, Coxiella burnetii, SARS-CoV-2 (COVID-19), etc.)
Website link
Status: Active
Biosafety Level 2 (or NBS2)
Moderate risk agents are handled for staff and the environment, so they are the most common in clinical, hospital and academic research.
Website link
Status: Active
Biosafety Level (or NBS1)
We work with agents who present a minimum danger to the laboratory staff and to the environment. Access to the laboratory is not restricted and the work is carried out regularly in standard laboratory benches, open work tables
Website link
Status: Active
GMO Type 1 – Zero or insignificant risk
USE CONFINED WITH GMO TYPE 1 (Zero or insignificant risk) IN SPAIN  In accordance with the provisions of article 5 of Law 9/2003, confined use is understood to be any activity for which the genetic material of an organism is modified or for which it, as modified, is cultivated, stored, used. , transport, destruction or disposal, provided that containment measures are used in carrying out these activities, in order to limit contact with the population and the environment.
Website link
Status: Active
GMO Type 2 – Low risk
USE CONFINED WITH GMO TYPE 2 (Low Risk) IN SPAIN  In accordance with the provisions of article 5 of Law 9/2003, confined use is understood to be any activity for which the genetic material of an organism is modified or for which it, as modified, is cultivated, stored, used. , transport, destruction or disposal, provided that containment measures are used in carrying out these activities, in order to limit contact with the population and the environment.
Website link
Status: Active
CERCA - Ginys