Flow cytometry platform
GINYS-IRSP-004
The flow cytometry platform provides users with the latest generation equipment for cytometric analysis and separation of cell populations. Unit staff support users in experimental design, data acquisition, and analysis of results. Furthermore, unit staff performs periodic training of the users to facilitate their non-assisted use of the equipment in the platform.
Services
The flow cytometry platform has a wide range of applications in flow cytometry and cell sorting and is open to innovation with new techniques at the request of researchers.
- Support users from experimental design to data analysis to perform proper flow cytometry
- Sample processing and analysis service for internal and external users
Conventional cytometry
- Characterization of cell populations by multicolor immunophenotyping.
- Essays of cell viability and proliferation in animal and bacterial cells.
- Essays of cell cycle and apoptosis.
- Gene expression (analysis of fluorescent proteins) and quantification of transfection efficiency.
- Magnetic column cell enrichment for the detection of minority populations.
Cell sorter
- Up to 4 cell subpopulations can be obtained simultaneously in a biosafety cabin.
- Automated magnetic cell separation of up to two populations.
High Throughput Screening (HTS)
- Analysis of gene expression and immunophenotype profiles, detection of DNA, RNA and protein in a single plaque.
- Possibility to include up to 50 analytes in the same plaque.
Equipment
Analyzer cytometer
- FACSCalibur (BD). 2 lasers: 4 fluorescence parameters.
- FACSCanto II (BD). 3 lasers: 8 fluorescence parameters.
- MACSQuant 10 (Miltenyi Biotec). 3 lasers: 8 fluorescence parameters. Equipped with a magnetic enrichment column.
- MACSQuant VYB (Miltenyi Biotec). 3 lasers: 8 fluorescence parameters. It is equipped with a yellow-green laser (561nm) and it specializes in bacteria and fluorescent proteins (GFP, mCherry, dTomato…).
- MACSQuant 16 (Miltenyi Biotec). 3 lasers: 14 fluorescence parameters. Specialized in nanocytometry.
Cell sorting
- FACSAria (BD) inside biosafety cabin. 3 lasers: 9 fluorescence parameters. Collection in cytometry tubes (up to 4 populations), plaque, or slides. Single-cell option.
- AutoMACS (Miltenyi Biotec). Automated magnetic separation.
Multiplex Analysis
- MAGPIX (Luminex). Analysis of up to 50 analytes per plaque.
Off-line analysis programs
- FACSDiva and Cell Quest Pro.
- Flow Logic- software for cytometric analysis (Miltenyi).
- Belysa Immunoassay Curve-Fitting Software (Luminex- Millipore).
Staff
Lia Ros Blanco | LRos@santpau.cat
| 932919000 Ext. 8083
Marta Soler | MSolerC@santpau.cat
| 606 52 67 76
Publications
Peptides against low density lipoprotein (LDL) aggregation inhibit intracellular cholesteryl ester loading and proliferation of pancreatic tumor cells
Endothelial Progenitor Cells Count after Acute Ischemic Stroke Predicts Functional Outcome in Patients with Carotid Atherosclerosis, Journal of Stroke and Cerebrovascular Diseases
