Flow cytometry platform
GINYS-IDIBELL-005
José Miguel Andrés Vaquero
Cap de plataforma
Our Flow Cytometry facility provides users with the state-of-the-art equipment for flow Cytometry analysis and high-speed cell sorting. Unit personnel support users for experimental design, data analysis and acquisition.
Following manufacturer instructions, a proper maintenance and standard quality control procedures are routinely applied to ensure best quality results.
Services
Services:
- Sample acquisition at the analyzer, either with technical support, or without, after receiving a proper training.
- Cell Sorting with technical support.
- Data analysis support if required.
The aim of the Cytometry Unit is to get ready and focus on the applications of interest of our users.
Most common applications that routinely are run:
Cell Sorting:
- In purification mode (when the most important is to get a high purity of the cell population of interest from the original sample). Up to 4 cell populations at the same time (4way sorting).
- In enrichment mode (when the most important is to get a high recovery of the cell population of interest from the original sample). So that, purity is slightly compromised. Available in 4-way sorting too.
- In single cell deposition (to get just one single cell or any desired number of cells within a single well using any of the standard multiwelll plates).
- In mixed mode (purify and enrich mode run simultaneously even for 2 cell populations at a time).
Analysis:
- Multicolor Immunophenotyping Characterization.
- Viability Assays.
- Cell Cycle and Proliferation Assays.
- Gene Expression (Fluorescent Protein analysis).
- Etc…
Equipment
The unit is equipped with 3 flow cytometers from Beckman Coulter Company:
- 1x High Speed Cell Sorter Moflo-XDP: 4 lasers (B-R-V-Y), 11 colors (5+2+2+2) o (5+2+4)
- 2x Gallios Analizers: 3 lasers (B-R-V), 10 colors (5+3+2).
Laser abbreviations and Optical Bench configuration:
Lasers:
V: Violet, 405nm; B: Blue, 488nm; Y: Yellow, 561nm; R: Red, 635nm.
Optical Bench Configuration:
10C [2 + 5 + 3] = 10 Colors or PMT detectors in total and [number of them linked to a specific laser line).
Publications
Molecular markers of putative spermatogonial stem cells in the domestic cat
Reprod Domest Anim Nov6
Reprod Domest Anim Nov6
Direct Conversion of Fibroblasts to Megakaryocyte Progenitors
Cell Rep 17(3):671-683.
Cell Rep 17(3):671-683.
Neuronopathic Gaucher's Disease: induced pluripotent stem cells for disease modelling and testing chaperone activity of small compound
Hum Mol Genet 22 (4): 633-45. Epub 2012 Oct 31.
Hum Mol Genet 22 (4): 633-45. Epub 2012 Oct 31.
Isolation and in vitro culture of primary cardiomyocytes from adult zebrafish hearts
Nat Protoc 8(4): 800-9.
Nat Protoc 8(4): 800-9.
Mcad-mediated intercellular interactions activate satellite cell division
J Cell Sci 126 (Pt 22):5116-31. Epub 2013 Sep 17.
J Cell Sci 126 (Pt 22):5116-31. Epub 2013 Sep 17.
AnnaFrances8/JOIN-Software-v1: Microscopy image processing program. (Version v1).
Anna Francès-Abellán. (2023). AnnaFrances8/JOIN-Software-v1: Microscopy image processing program. (Version v1). Zenodo. https://doi.org/10.5281/zenodo.8370192
Anna Francès-Abellán. (2023). AnnaFrances8/JOIN-Software-v1: Microscopy image processing program. (Version v1). Zenodo. https://doi.org/10.5281/zenodo.8370192