Biobank and immunohistochemical platform
GINYS-IRSP-003
Esther Peña Sendra
Coordinadora
The Biobank and Immunohistochemistry Platform is a platform to support biomedical research, whose main function is the processing of biological samples according to quality criteria.
Its mission is to obtain, process, store, manage and make available to the scientific community human biological samples and associated data, in accordance with agreed standard working procedures, as well as maintaining the traceability of the sample. and its associated data, in order to promote quality biomedical research, respecting the rights of donors.
The aim of the IIB Sant Pau Biobank is to increase the accessibility of human biological samples to applicant researchers so that they can use them in their research projects, provided that they meet the ethical and legal requirements required by current legislation.
For its part, the Immunohistochemistry laboratories of the Biobank and Immunohistochemistry Platform offer biological sample processing services, both of human origin and animal models, for analysis using histological techniques, from sample processing, cutting and staining until digitization of preparations, histological analysis and delivery of results. The Platform offers the possibility of developing new histological methods and staining, according to the demand and needs of our users, both internal and external. In addition, training is provided to users so that they can use the platform's equipment in self-service mode.
Services
In addition to the catalogs of human biological samples, the Biobank and Immunohistochemical Platform offers a wide range of services:
BIOBANK
- Management of requests for biological material for research purposes
- Ethical-legal advice on the creation of new collections
- Scientific-technical advice on the treatment of biological samples
- Processing of human biological samples: peripheral blood, bone marrow, urine, frozen tissue, etc.
- Conservation and custody of human biological samples
- Cryopreservation of viable cells
- Management of sample collections and associated data
- Nucleic acid extraction from different types of biological samples
IMMUNOHISTOCHEMISTRY
- Tissue decalcification
- Tissue processing for paraffin embedding and O.C.T.
- Section of paraffin blocks and O.C.T.
- Antigen retrieval
- Automatic, single and double immunostaining
- Stains: hematoxylin-eosin, giemsa, oil red, trichrome, PAS, Wright’s stain, alcian blue, safranin, sirius red, etc…
- Preparation of tissue microarrays (TMA)
- Fluorescent in situ hybridization (FISH) on tissue
- Digitization of histological preparations, under bright field, fluorescence and FISH conditions
- Qualitative and quantitative histological analysis
The Platform offers the possibility of implementing new histological methods and stains, according to the demand and needs of our users, both internal and external.
Equipment
Equipment
- 15 Ultralow freezers -80ºC NUAIRE NU-9668E with double-emergency system alarm. 24h monitoring
- Liquid nitrogen tank CHART MVE 1211 with automatic filling
- Vapour nitrogen tank CBS V-5000 EHAB with automatic filling
Biosafety BSL-2 Laboratory
- Biosafety cabinet IIA NUAIRE
- Refrigerated centrifuge HERAEUS MEGAFUGE 40R
- Refrigerated centrifuge KENDRO LABOFUGE 400R
- Refrigerated centrifuge EPPENDORF 5415R
- Cell counter BIORAD TC-20
- Nucleic acid extractor QIACUBE
Immunohistochemistry new IR-HSCSP building
- Tissue processor SAKURA TISSUE TEK VIP 5 JR
- Microwave Tissue processor KOS
- Paraffin Embedding Module SAKURA TISSUE TEK TEC5
- Cold plate for Paraffin Modules LEICA ARCADIA C
- Rotary Microtome MICROM HM355S.
- Paraffin section flotation bath SELECTA TERMOFIN.
- Drying oven TERMARKS B8000
- Cryostat LEICA CM1950
- Immunohistochemistry automatic stainer AGILENT AUTOSTAINER AS48
- Automatic Stainer for histologic staining SAKURA TISSUE TEK PRISMA
- Semiautomatic tissue array GALILEO CK3500.
- Fume hood NUAIRE NU-S813-400E
- Fume hood NUAIRE NU-164-424E
- Scanner Panoramic Scan II (3DHistech)
Immunohistochemistry Lab Pavilion 11
- Paraffin Embedding Module LEICA EG1150H: assisted and non-assisted
- Cold plate for Paraffin Modules LEICA EG1150C: assisted and non-assisted
- Rotary Microtome LEICA JUNG RM2055: assisted and non-assisted
- Paraffin section flotation bath LEICA HI1210: assisted and non-assisted.
- Drying oven MEMMERT BE 300
- Cryostat LEICA JUNG CM3050S: assisted and non-assisted
- Fume gard LABORSYSTEM
- Scanner Panoramic MIDI BF.
- Workstation for qualitative and quantitative histological analysis
Staff
J. Oriol Juan Babot | OJuan@santpau.cat
| +34 935565889 | ORCID
Sabina Cisa Wieczorek | SCisa@santpau.cat
| 932919000 Ext. 8037
Iris Rodríguez Rodríguez | IRodriguezR@santpau.cat
| 932919000 Ext. 8037
M. Angels Velasco Murcia | AVelascox@santpau.cat
| 932919000 Ext. 1426
