Cell immortalization
GINYS-IJC-007
Carolina De La Torre
The Cell Immortalization Unit of the Josep Carreras Leukemia Research Institute offers B-cell infection with Epstein-Barr virus (EBV) which leads to further and subsequent immortalization. This is considered the method of choice for generating lymphoblastoid cell lines (LCL). Cell culture is an essential tool for studying the fundamentals of genetic background variables. With the development of personalized medicine, this is increasingly applied to the development and safety testing of drugs.
Immortalized cells are derived from primary cells, thus establishing an immortalized cell line to achieve cultured cells in vitro. The purpose of unlimited proliferation and no difference between cells.
The Cell Immortalization Unit works closely with you in an integrated manner starting with the project discussion (including the project outline and experimental design). we are continuously working to optimize standard operating procedures and implement the latest methodologies.
With our personalized service, you can get our high quality customer service experience with high success rates, frequent communication and fast deadlines.
Services
- Cell immortalization service to generate cell lines of lymphocytes
- Produce control material for rare genetic disorders
- Cryopreservation service to obtain sufficient material from DNA, RNA, and protein samples, which appears to be a valid strategy for further studies.
Staff
Carolina De La Torre | cdelatorre@carrerasresearch.org
| 935 572 800 (Mon-Fri 08.30-17.30) | ORCID
| PRC Page
Publications
The impact of MECP2 mutations in the expression patterns of Rett syndrome patients
Hum Genet. 2005 Jan;116(1-2):91-104
Hum Genet. 2005 Jan;116(1-2):91-104
Epigenetic inactivation of the Sotos overgrowth syndrome gene histone methyltransferase NSD1 in human neuroblastoma and glioma
M. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21830-5
M. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21830-5
A novel Werner Syndrome mutation: pharmacological treatment by read-through of nonsense mutations and epigenetic therapies
R. Epigenetics. 2015;10(4):329-41
R. Epigenetics. 2015;10(4):329-41
A mutation in the POT1 gene is responsible for cardiac angiosarcoma in TP53-negative Li-Fraumeni-like families
J. Nat Commun. 2015 Sep 25; 6:8383
J. Nat Commun. 2015 Sep 25; 6:8383
Whole-exome sequencing identifies MDH2 as a new familial paraganglioma gene
J Natl Cancer Inst. 2015 Mar 11;107(5):djv053
J Natl Cancer Inst. 2015 Mar 11;107(5):djv053
Germline missense pathogenic variants in the BRCA1 BRCT domain, p. Gly1706Glu and p. Ala1708Glu, increase cellular sensitivity to PARP inhibitor olaparib by a dominant negative effect
Hum Mol Genet. 2016 Dec 15;25(24):5287-5299
Hum Mol Genet. 2016 Dec 15;25(24):5287-5299
Germline mutations in the spindle assembly checkpoint genes BUB1 and BUB3 are infrequent in familial colorectal cancer and polyposis
Mol Cancer. 2018 Feb 15;17(1):23
Mol Cancer. 2018 Feb 15;17(1):23
