High resolution mass spectrometry
GINYS-ICIQ-003
Dr. Noemí Cabello
Gerent
The High Resolution
Mass Spectrometry Unit (HRMS) offers access to
state-of-the-art mass spectrometry instruments capable of characterizing the
wide range of compounds that are usually of interest to the Institute. The
apparatus includes most of the ionization options, introduction of
chromatographic samples and techniques that are necessary to solve problems of
mass spectrometry in chemistry. In addition to the characterization of pure
samples, the unit also supports research projects that depend more on mass
spectrometry experiments, such as mechanical studies, intermediate
identification, characterization of impurities or biochemical applications. In
addition, the Unit offers a comprehensive training to guarantee an optimal use
of the installations and an adequate interpretation of the data.
Services
Autoflex™ mass spectrometer: MALDI-TOF-MS
m/z up to 70,000
Peptides and
Proteins, Polymers, Oligonucleotides
Proteins, Polymers, Oligonucleotides
Low
molecular weight molecules
molecular weight molecules
MaXis Impact™mass spectrometer (Bruker
Daltonics) with ESI and APCI ionization sources. Coupled to an Acquity™ UPLC (Waters).
Daltonics) with ESI and APCI ionization sources. Coupled to an Acquity™ UPLC (Waters).
ESI-QqTOF-MS
ESI: Polar
molecules, high and low molecular weight compounds.
molecules, high and low molecular weight compounds.
Proteins
Organometallics.
Non-Covalent
Interactions
Interactions
MS/MS analysis.
APCI- QqTOF-MS
Medium
Polarity Compounds, m/z up to 1500
Polarity Compounds, m/z up to 1500
MicroTOF™ mass spectrometer (Bruker Daltonics)
with ESI, APCI and Coldspray ionization sources. Coupled to an Ultimate3000™
HPLC (Thermo Scientific).
with ESI, APCI and Coldspray ionization sources. Coupled to an Ultimate3000™
HPLC (Thermo Scientific).
TOF-MS
Coldspray ionization:
analysis of ionic compounds at low temperature (until -100 ºC).
analysis of ionic compounds at low temperature (until -100 ºC).
Labile species,
organometallic compounds, reaction intermediates.
organometallic compounds, reaction intermediates.
ESI/MALDI dual source and Coldspray Ionization
coupled to ion mobility (IM)
coupled to ion mobility (IM)
Coldspray ionization-Ion
Mobility-MS: Unique combination for the analysis of ionic compounds at low
temperature (up to -100 ºC), including separation of isomers.
Mobility-MS: Unique combination for the analysis of ionic compounds at low
temperature (up to -100 ºC), including separation of isomers.
MALDI
Imaging – Ion Mobility-MS: Isomers separation / Isobaric ions. Localization of
ions on tissues.
Imaging – Ion Mobility-MS: Isomers separation / Isobaric ions. Localization of
ions on tissues.
Equipment
- Elute UHPLC-timsTOF flex™ mass spectrometer (Bruker Daltonics) ESI/MALDI dual source and Coldspray Ionization coupled to ion mobility (IM).
- Coldspray ionization-Ion Mobility-MS: Unique combination for the analysis of ionic compounds at low temperature (up to -100 ºC), including separation of isomeric ions.
- MALDI Imaging – Ion Mobility-MS: Isomers separation / Isobaric ions. Determination of ions on surfaces.
- Liquid chromatograph coupled time-of-flight mass spectrometer
Waters Alliance HPLC coupled to MicroTOF Focus (Bruker Daltonics) with electrospray (ESI) and atmospheric pressure chemical ionization (APCI) options. - Liquid chromatograph coupled time-of-flight mass spectrometer
Waters LCT Premier liquid chromatograph coupled time-of-flight mass spectrometer (HPLC/MS-TOF) with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) options. - MALDI time-of-flight mass spectrometer
- BRUKER Autoflex matrix assisted laser desorption ionization (MALDI) time-of-flight mass spectrometer.
Liquid chromatograph coupled quadrupole-time-of-flight mass spectrometer. (UPLC-QqTOF). - Waters liquid chromatograph (UPLC) coupled to MaXis Impact (Bruker Daltonics) hybrid analyzer: quadrupole-time-of-flight mass spectrometer (UPLC/MS-QqTOF) with electrospray ionization (ESI) and atmospheric pressure chemical ionization (APCI) options.
Staff
Carly Chedotal | cchedotal@iciq.es
| 977 920 200 (ext. 302)
Vanessa Martínez | vmartinez@iciq.es
| 977 920 200 (ext. 302)
