Cytometry
Institut d’Investigació Sanitària Pere Virgili
Parc Sanitari Joan XXIII
C/ Doctor Mallafrè Guasch, 4
43005 Tarragona
Services
- 01 Analysis of cell subpopulations
- 02 Apoptosis
- 03 Cell cycle, proliferation
- 04 Functional analysis
- 05 Viability
- 06 Cell separation
- 07 Advice and training for users of the platform
Equipment
The platform has a FACS Aria III (BD), a high-speed, high-resolution particle and cell flow analyzer and separator. The current configuration has 4 lasers with a total of 18 fluorochromes. In addition, it also has an autoMACS magnetic cell separator. The analysis software is FACSDiva (BD) and FlowJo. The cell classifier-separator is managed by facility personnel, who also support users in experimental design and data analysis. Analyzers can be handled by the users themselves after a training session. Installation staff assists with experimental design and oversees experiments when necessary. We recommend that you discuss the experiments with the facility staff in advance to properly design the experiments.
Equipment:
Thermo Shandon Processor (Patchcentre), Shandon Histoccentre 2 (Shandon), Microtome RM 2255 (Leica), Wax Stove, Varistain 24-4 Hematoxylin Dye (Shandon), Arraymold for Multi-Woven Matrix Construction, Digital Slide Scanner, Image analysis software Image Pro-Plus 5.0, Scancope, Image J, Matlab.