Cytometry
GINYS-IDIBAPS-003
Isabel Crespo
Cap de plataforma
CEK Building
Rosselló, 149-153
Basement Floor
08036 Barcelona
The Cytometry and Cell Sorting Facility has
the mission of making available to the research community cutting-edge
technologies and technical personnel specialized in the field of flow cytometry
and cell sorting, offering its services both to IIS-IDIBAPS personnel and to
other external entities, especially R&D centers, public or private.
To do this,
we have a 62m2 cytometry room in which 3 digital cytometers and 1 analog cytometer
are located, and a 44m2 cell sorting room with 2 digital cell sorters.
The Facility
staff consist of a coordinator, a senior technician and 2 junior technicians;
all of them full-time, organized so that the Facility works uninterruptedly
from 8h to 20h, to assimilate as many experiments as possible. The staff has
proven experience in the field of flow cytometry and cell sorting.
We offer a
wide range of applications in flow cytometry and develop or implement new ones
depending on researchers needs.
We provide
training, as well as technical knowledge and advice for experimental design, analysis,
and data management.
Services
Services for IIS-IDIBAPS and other external entities,
public or private.
public or private.
All services can be done with the assistance of the Facility
staff or unassisted.
staff or unassisted.
·
Sample analysis by flow cytometry (up
to 5 LASER and 18 fluorescence parameters). With HTS module for plate reading.
Sample analysis by flow cytometry (up
to 5 LASER and 18 fluorescence parameters). With HTS module for plate reading.
·
Cell sorting (up to 5 LASER and 18
fluorescence parameters). Collection in tubes (up to 4 populations), plates or slides.
Index single_cell sorting for sequencing (96 and 384 well plates).
Cell sorting (up to 5 LASER and 18
fluorescence parameters). Collection in tubes (up to 4 populations), plates or slides.
Index single_cell sorting for sequencing (96 and 384 well plates).
·
Data analysis (DiVa 9 , CellQuestPro
6.0.3, ModFitLT 3.2, FlowJo X, FCAPArray 1.0.1, SpectroFlo, FCS Express).
Data analysis (DiVa 9 , CellQuestPro
6.0.3, ModFitLT 3.2, FlowJo X, FCAPArray 1.0.1, SpectroFlo, FCS Express).
·
Training for using bench-top
cytometers unassisted.
Training for using bench-top
cytometers unassisted.
·
Experimental advice on project/assay development and
experimental design.
Experimental advice on project/assay development and
experimental design.
Equipment
. BD FACSCanto II digital bench-top
cytometer: 3 LASER, 8 fluorescence parameters.
cytometer: 3 LASER, 8 fluorescence parameters.
·
BD LSRFortessaSORP digital bench-top
cytometer: 5 LASER, 18 fluorescence parameters.
BD LSRFortessaSORP digital bench-top
cytometer: 5 LASER, 18 fluorescence parameters.
·
BD LSRFortessaSORP digital bench-top
cytometer with plate reader: 4 LASER, 16 fluorescence parameters.
BD LSRFortessaSORP digital bench-top
cytometer with plate reader: 4 LASER, 16 fluorescence parameters.
·
BD FACSARIA II digital cell soter: 3
LASER, 9 fluorescence parameters.
BD FACSARIA II digital cell soter: 3
LASER, 9 fluorescence parameters.
·
BD FACSAriaSORP digital cell sorter: 5
LASER, 18 fluorescence parameters.
BD FACSAriaSORP digital cell sorter: 5
LASER, 18 fluorescence parameters.
.Cytek_AURORA full spectrum flow cytometer: 5 LASER, Plate Loader, 40 tubs rack.
Staff
Cristina López García | clopezg@recerca.clinic.cat
| 932 275 400 Ext.3364
Sara Ozcoz | ozcoz@recerca.clinic.cat
| 932 275 400 Ext.3364
Joel Martínez | JMARTINEZO@recerca.clinic.cat
| 932 275 400 Ext.3364
