Advanced light microscopy unit
GINYS-CRG-001
It is the aim of this unit to provide instruments covering the whole application spectrum of advanced light microscopy and to make them available to researchers from the CRG as well as for visitors from other research institutions.
As Core Facility for light microscopy, the unit provides a number of advanced light microscopy systems, equipment for sample preparation and maintenance prior to imaging and resources for the subsequent processing of the image data.
The staff assists researchers in the experimental planning of light microscopy experiments and provides the in-depth training for the operation of the microscopes and for specific imaging techniques. Additionally, support is provided in the processing, rendering and analysis of the acquired datasets. If needed, custom analysis routines will be designed.
Services
- Consultation and assistance starting from experimental design, sample preparation, imaging, and data analysis
- Technology training on the operation of the unit’s microscope systems
- Support for experiments requiring advanced imaging methods
- User access to state-of-the-art microscopy systems
- User access to analysis workstations
- Image data analysis services
Equipment
The equipment offer is available to trained users and covers all current imaging applications with high-end imaging systems.
Super-resolution microscopy
- Leica confocal STED 3X FALCON system with white light laser (WLL) and three depletion wavelengths (WLL: 470 – 670 nm, 592nm CW, 660nm CW, 775nm pulsed)
- Nikon N-STORM (405nm, 488nm, 561nm, 647 nm lasers)
- Zeiss LSM 980 with airyscan 2 (405, 488, 561, 639 nm lasers)
- Leica confocal CW STED super-resolution microscope with 592 nm CW depletion wavelength
- Leica Ground State Depletion (GSD) microscope system (488 nm, 532 nm, 647 nm, 405 nm backpumping)
Multiphoton microscopy
- Leica inverted confocal/multiphoton system (405, Argon ion, 561, 633nm and Mai Tai DeepSee)
- Leica upright confocal/multiphoton system (405, Argon ion, 543, 633nm and Mai Tai DeepSee)
Confocal Microscopy
- Leica STELLAIRS confocal system with white light laser ( WLL: 485 – 685, also 405, 488, 561 nm lasers)
- Leica inverted confocal system (405, 458,476,488,496,514,561 and 633 nm lasers)
- Leica inverted confocal system for live-cell imaging (458,476,488,514,543 and 633 nm lasers)
- Andor Dragonfly Spinning Disk confocal system (Borealis illumination, 488 and 561 nm lasers)
- Andor Revolution XD Spinning Disk confocal microscope (405, 445, 488, 514, 561 nm lasers)
Widefield Microscopy
- Zeiss Cell Observer fluorescence microscope for live-cell imaging
- Olympus MVX10 Macro Zoom fluorescence microscope
Screening Microscopy
- Molecular Devices ImageXpress Micro Purchase year: 2009
Computer resources
- AQUIFER HIVE for image processing and analysis
- 2 workstations for image processing
Workstations and Software packages
- Analysis workstations and AQUIFER HIVE for image processing and analysis
- Open source software: ImageJ/Fiji, Cel Profiler, Advanced Cell Classifier
- SVI Huygens Deconvolution software
- Bitplane Imaris 3D and 4D imaging software
Staff
Publications
Müller glia fused with adult stem cells undergo neural differentiation in human retinal models
Ebiomedicine. 77103914-103914 - 2022-01-01 77(),
Ebiomedicine. 77103914-103914 - 2022-01-01 77(),
Stimulated emission depletion (STED) super resolution imaging of RNA- and protein-containing domains in fixed cells
Methods. 18768-76 - 2021-03-01 187(),
Methods. 18768-76 - 2021-03-01 187(),
Photobleaching and Sensitized Emission-Based Methods for the Detection of Förster Resonance Energy Transfer
Methods In Molecular Biology. 2040235-274 - 2019-01-01 2040(),
Methods In Molecular Biology. 2040235-274 - 2019-01-01 2040(),
TANGO1 assembles into rings around COPII coats at ER exit sites
JOURNAL OF CELL BIOLOGY. 216 (4): 901-909 - 2017-04-01 216(4)
JOURNAL OF CELL BIOLOGY. 216 (4): 901-909 - 2017-04-01 216(4)
Mammalian HP1 Isoforms Have Specific Roles in Heterochromatin Structure and Organization
Cell Reports. 21 (8): 2048-2057 - 2017-01-01 21(8)
Cell Reports. 21 (8): 2048-2057 - 2017-01-01 21(8)
