Proteomics
GINYS-IJC-006
Carolina De La Torre
Unitat de Proteòmica
Edifici IJC, Campus ICO-Germans Trias i Pujol
Ctra de Can Ruti, Camí de les Escoles s/n
08916 Barcelona, Spain
The Proteomics Unit of the Josep Carreras Leukemia Research Institute has been created to support the scientific community that offers mass spectrometry services to the academic and private sector.
One of the main objectives of the unit is to offer a high quality service in the following disciplines: descriptive proteomics, quantitative proteomics (SILAC, TMT, Label Free), identification of biomarkers through the main human diseases, protein characterization and identification of post-translations. modifications (phosphorus and phosphorus enrichment, acetyl and acetyl enrichment, ADP ribosylation), identification and quantification of protein-protein interactions, and identification and additional biological validation of bioactive peptides with potential applications in human health care, including food supplements and cosmetics, among others.
The proteomics unit, in an effort to implement the philosophy of the Josep Carreras Leukemia Research Institute, works to promote the incorporation of proteomics as a necessary tool for the development of clinical and basic projects in our center. In this line, the implementation of state-of-the-art services allows clinical and basic researchers to apply these tools to personalized medicine.
Services
Anàlisis de proteòmica LC-MSMS
- Identification of proteins from a single band
- Protein identification and quantification of complex samples – iTRAQ-based proteomic analysis – TMT-based proteomics analysis – SILAC-based proteomic analysis – Label-free quantification – Parallel reaction monitoring (PRM) – SRM and MRM
- Post-translational modification analysis of proteins: – iTRAQ-based proteomic analysis – TMT-based Proteomics Analysis –
- SILAC-based proteomic analysis – Label-free quantification – Parallel reaction monitoring (PRM) – SRM and MRM
- Protein-protein interaction networks: – Co-immunoprecipitation (co-IP) – Analysis of crosslinking protein interaction – Pull-down assay
- Post-translational modification analysis of proteins: – Phosphorylation (phospho-enrichment and identification of the phosphorylation site) – Acetylation (enrichment of acetyl-lysine antibodies) – Methylation – ADP-ribosylation
Bioinformatics Service for proteomics
- Functional annotation and enrichment analysis
- Clustering analysis
- Network analysis
- Statistical analysis
- Proteomic analysis of post-translational modifications
Applications of proteomics
Identification and validation of biomarkers in different types of samples:
- Cells.
- Fluids (saliva, serum, plasma, urine, interstitial fluids, etc.)
- Tissues (muscle, brain, lung, kidney, heart, retina, etc.)
- Fecal
- Exosomes
- Secretoma
- Mat.risome
Staff
Carolina De La Torre | cdelatorre@carrerasresearch.org
| 935 572 800 (Mon-Fri 08.30-17.30) | ORCID
| PRC Page
